Malaria has been reported to be one of the most debilitating illnesses of all time and is the most common parasitic disease in sub-Saharan Africa. Methanol extract of the four parts of Ficus sycomorus plant (leave, fruits, stem-bark and roots) were screen for in vitro anti-plasmodial activity using Plasmodium falciparum. The IC50 revealed that the fruits and leave extract have an IC50 ˃ 186µg while stem-bark and roots were discovered to be the most active with IC50 of 20.4µg. In vitro anti-plasmodial activity of organic solvent extracts (Hexane, ethylacetate and saturated butanol) of the methanol root extract revealed IC50 of 40µg, 20.4µg and 20.4 µg respectively. In vivo anti-plasmodial activity of the saturated butanol extract of the root was investigated in albino mice. Thirty (30) mice were inoculated with the parasitized donor erythrocytes containing Plasmodium berghei. One week after parasite inoculation, the animals were randomly distributed into six groups of five mice each. Group 1 served as negative control (not treated), groups 2 to 5 were experimental groups and were administered 20%, 40%, 60% and 80% of the LD50 (31mg, 62mg, 93mg and 124mg), while group 6 served as positive control (treated with artesunate). The mice were treated orally for seven consecutive days once daily. The results showed that the saturated butanol has anti-plasmodial activity in mice with percentage parasitemia inhibition of 50.6%, 80.8%, 100%, 100% and 100% for groups 2 to 5 and artesunate respectively. The results suggest that the plant have potential for the development of a novel anti-malarial agent.

Full mouth rehabilitation always requires meticulous treatment planning and careful attention for procedures. All the efforts are directed towards reestablishing a state of functional efficiency in which, the teeth and their periodontal structures, the muscles of mastication and temporomandibular joint mechanisms are all functions together in synchronous harmony. This clinical report describes the oral rehabilitation of a patient with multiple missing and decayed teeth. The specific treatment objective being restoration of masticatory function, esthetic rehabilitation and improved self confidence.

Many of mosquito genera are exophagic that bite humans outdoors. Most community members in Nigeria are in the habit of staying outdoors at dusk for relaxation which can expose them to mosquito bites. This necessitated the design of this study with the objectives to examine the distribution of exophagic mosquito genera in six communities in Ekiti State, Nigeria. The communities were selected through a multistage sampling method. Adult mosquitoes were collected outdoors from the six communities by using miniature Centre for Disease Control light traps for twelve months. The collected mosquitoes were sorted into sexes and genera. Data were analyzed by descriptive statistics and chi-square using SPSS. Five hundred and five (505) adult mosquitoes were collected outdoors in the six study communities, females (96.6%) and males (3.4%). Mosquito genera collected were Culex (90.5%), Anopheles (5.3%) and Aedes (4.2%). Culex and Aedes Population peaked in October but Anopheles population peaked in July. Availability of mosquito vectors at dusk in these study communities is an indication that the community members could be at risk of mosquito borne diseases.

Background: Globally, it is known that heath care workers particularly in critical care settings are at higher risk of occupational exposure to infections. Surveillance systems to monitor body fluid exposure have been established in developed world. However, such systems are not available and consequently, exposure to body fluids is rarely reported, documented and monitored in many African countries. Objectives: To assess knowledge, practice and factors influencing adherence of Universal Precautions of infection prevention among nurses at Muhimbili national hospital and Muhimbili orthopaedic institute Dar es salaam Tanzania. Design: Quantitative cross-sectional and observational study. SPSS was used for data analysis. Chi-square test and student t-test were used for analysis of categorical and continuous variables respectively and regression analysis were done to determine any significance. Participants: 144 nurses in intensive care units, emergency rooms and recovery rooms were assessed using a structured self-administered questionnaire and an observational checklist to 42 of 144. Findings: 38% of nurses had good knowledge of universal precautions. 85% reported to always wash hands, however, 34.3% of participants were observed to wash hands before putting on gloves. 94.4% reported to always use gloves, 67.4% reported to always wear a gown and 55.6% reported that they always wear a mask. Regarding sharps management 93.8% reported good practice and 95.2% demonstrated good sharps management practice. Sex and level of education of an individual significantly influenced the hand hygiene practice, working station and perceived severity significantly influenced the practice of wearing protective gear. Training on infection prevention and control significantly influenced sharps handling (All p<0.05). Discussion: Adherence of universal precautions were sub optimal. Reported and observed practice were incompatible as in other studies except in the practice of sharps management which observed results outweighed the reported results. Conclusion: Adherence to universal precaution is variably poor. Surveillance systems to monitor exposures to body fluids is required.

The small saphenous vein (SSV) has several termination modalities and several types of afferent branches. The latter participate in the physiopathology of insufficiency of the small saphenous vein and explain the majority of recurrences after open or endovenous surgical cures. This study details the modes of termination and topography of afferents of the SSV. This is a cadaveric dissection involving 62 members of fresh and embalmed anatomical subjects. A popliteal approach and an extension is made towards the femoral region and towards the lateral malleolus are performed. Several parameters have been studied: the number of SSV, the number and distribution of terminal branches in the popliteal vein, in the great saphenous vein or in the thigh and the existence of musculocutaneous afferents of leg and thigh. We found that 45% of terminations were with a sapheno-popliteal junction. This form was absent in 55% of the cases. Consequently, the termination was either in the great saphenous vein, or in the posterior muscular compartment of the thigh, or in integumentary branches of the leg or thigh, or in the twin veins. This SSV received, in its course, twin veins, femoral veins, and integumentary veins.These anatomical variations of termination make a rigorous clinical and ultrasonographic examination indispensable in order to have a good mapping of the vein an to avoid recurrence of varicoses after surgery.

L'Administration Fiscale a pour rôle de mobiliser les ressources fiscales qui sont prévues par la Loi des Finances d'un Etat. Cette mobilisation se réalise par la mise en exécution, par l'Administration Fiscale, des actes législatifs et réglementaires régissant le prélèvement fiscal. Ces derniers, constituant la législation fiscale, prévoit non seulement des prérogatives de l'administration fiscale mais aussi des droits et des obligations fiscales pour les contribuables. Pour ce faire l'administration fiscale doit pouvoir réaliser le contrôle fiscal dans le stricte respect des procédures fiscales, prévues dans la loi n°004/2003 du 13 mars 2003 telle que modifiée et complétée à ce jour, de façon à garantir un prélèvement juste de l'impôt et aussi d'entretenir des bonnes relations avec les contribuables. Etant donné que ces derniers doivent être considérés comme des partenaires et non des esclaves fiscales. Ainsi, nous pouvons affirmer ce qui suit : une administration qui vous simplifie la vie trouvera en face un contribuable citoyen ; une administration qui respecte les personnes et le droit trouvera en face un contribuable coopératif et une administration équitable trouvera en face un contribuable loyal.

Biotechnology is the emerging field of science that involved for the development of medical products medicines like vaccine based on DNA, recombinant vaccine genomics and proteomics. The purpose of this review included applications of biotechnology in healthcare. Several diseases remain undiagnosed at molecular level. By taken advantage through applications of biotechnology, many advancement has been taken in the in the field of medicine and health sciences. These medicines are alternative of traditional medicines which were used without any diagnosis. In PCR, amplification of gene product visualize in a specific manner. In gene therapy affected cells are replaced with normal cells it can be used for both somatic cells and germ cells. Fluorescence in situ hybridization has important applications to determine that how chromosomes arrangement involved in the behavior of an individual. Biotechnology also has other important applications in healthcare such protemics , genomics and gene therapy. Several vaccines have been made through using tools of biotechnology. There are some diseases that needed to treat at genetic level though healthcare biotechnology.

Dental implants are popular treatment option for replacement of missing teeth. Teeth loss causes alveolar bone resorption. Implant placement may require sufficient bone volume. Therefore bone augmentation can be done prior to implant placement. For bone augmentation autograft, allografts, xenografts and synthetic materials can be used. After augmentation implants can be placed successfully. In this case report autogenous block graft is taken for augmentation. Rehabilitation of edentulous region is done by implant placement.

Short guide RNAs of catalytically inactive CRISPR associated 9 nuclease uses to express endogenous genes in human cells and bacteria. Chimeric nucleases help in precise and efficient genetic modifications leads to double strand breakage which can be repaired by DNA repair mechanism by homology directed repair and non-homology end joining. Here, we describe that single or multiple gRNAs direct dCas9 fused to a VP64 transcriptional activation domain to enhance the expression of endogenous human genes. Light-activated CRISPR-Cas9 effector (LACE) system induces dynamic regulation of genes in the presence of blue light. Targeted activation of endogenous neurogenic genes in transgenic mice leads to direct and efficient conversion of astrocytes into functional neurons in vivo. CRISPR-on can activate the endogenous IL1RN, SOX2, and OCT4 genes. Activation of genes was achieved by clusters of 3-4 sgRNAs that bind to the proximal promoters, result in their synergistic action in gene induction. Moreover, it fused with transcriptional activators in endogenous human INS (silenced gene with a fully methylated promoter). Multiplexed gene editing helps in metabolic engineering in yeast and CHO mammalian host. In this review we describe the applications and development of Cas9 for a variety of research or translational applications.

Goal of prosthodontics is rehabilitation of missing oral and extra oral structures with restoration of normal function of mastication, speech, swallowing, esthetics etc. Defects of orofacial region, whether congenital or acquired, affects the patient physically and psychologically leading to poor quality of life. Benign and malignant lesions in the oral region are common, which requires surgical corrections. Surgical intervention of such lesions results in defects, affecting the function and esthetics of an individual. This case report explains prosthodontic rehabilitation of maxillectomy defect caused due to surgical excision of Ameloblastoma with Hollow bulb obturator with Cast partial denture. By fabrication of this prosthesis it covers the anatomic defect which forms communication among the oral cavity, nasal cavity and maxillary sinus.

COVID-19 infection is the current challenge that faces the research community worldwide. Although chloroquine phosphate, an old drug for management of malaria, revealed to have clear value and tolerable safety against COVID-19 associated pneumonia in multicenter clinical trials conducted in China, we theorize that minocycline combined with suitable non-steroidal anti-inflammatory drug will give better result than Chloroquine phosphate monotherapy. Nouraldein theory needs invitro and Invivo experiments to confirm it.

This study aims to look at the effect of manager ownership, manager quality, and conservatism on earnings quality in banking companies in Indonesia. The population used in this study is banking companies listed on the Indonesian stock exchange. The sample used was 41 banking companies on the Indonesia Stock Exchange in the period 2013-2018. Purposive sampling is used in this study, based on sampling criteria, the number of observations was 218 unit of analysis. The results of the study found that manager ownership has a positive and significant effect on the quality of accrual earnings in companies in the banking sector, while manager quality and conservatism do not have a significant effect on quality accrual earnings in a sample of banking sector companies in Indonesia.

Sinusitis one of the most troublesome diseases disturbing the usual daily activities. It is manifested by severe headache, nasal discharge, sneezing etc which creates a great problem. For its management analgesics, nasal decongestants & finally curettage of the sinus to remove the fluid deposit is adopted in modern medical science. However in Ayurveda Nasya & Dhumapan is best applied in this connection. A clinical trial was carried out to evaluate the effectiveness of Sadvindu Taila nasal drop in the management of frontal sinusitis. For this purpose total 40 patients of frontal sinusitis were collected from the OPD & IPD of Gangasheel Ayurvedic medical college, Bareilly(UP) as per the selection criteria. They were divided into two groups i.e. trial group & control group (consisting of 20 patients each) to whom the nasal drop Sadvindu Taila & Otrivin (Xylometazoline hydrochloride) nasal drop was given respectively. The trial drug was given in the prescribed dose for 7 days under Nasya & Dhumapana procedure. The control drug, in normal way only nasal instillation. After 7 days evaluation was done depending on the subjective & objective parameters. For statistical evaluation, mean ± SD, paired t-test was calculated. It was observed that the trial drug is very much effective in decreasing Headache, sneezing, nasal discharge & other allergic manifestation. Radiological the fluid level also decreased. Though the drug is better effective but it is not accepted by all the patients as it causes severe irritation in nasal mucosa. With the total study it can be concluded that the trial drug Sadvindu Taila has a great scope in the management of sinusitis, especially frontal sinusitis. Further studies are required to establish the drug more scientifically.

The Total Aeromagnetic Data covering the study area was subjected to Spectral Depth Analysis and Source Parameter Imaging (SPI).The entire area was divided into nine sections. Spectral Depth Analysis was run for each of these nine sections. The result from Spectral Depth Analysis shows that a maximum depth of 3.174km was obtained around Konkoso in Sheet number 117 of the study area. Minimum depth estimates of 0.586 km were observed around the basement regions. Result obtained from the Source Parameter Imaging (SPI) method has its highest sedimentary thickness of about 2.4 km to 3 km in areas around Kaoje towards the central part up to around Konkoso in the southern part. The shallow sedimentary thickness was found predominantly around Shanga in the northeastern (NE) part, up to around Yelwa in the southeastern (SE) part. Geophysical implication of the result to oil and gas exploration in the area is briefly discussed.

The nature has provided an entire warehouse of remedies to cure ailments of mankind. About 80 per cent of the world's population depends partially or wholly on traditional medicine for its primary health care needs. Herbal medicines as the key remedy in traditional medical system have been used in medical practice for past era and have made a great involvement to maintain human health. Thus Pharmacognostical study appears to be of enormous value in identification of commercial samples of the market to find their authenticity and establishing identity of adulterant or substituent. In addition lack of standardization, dishonest commercial practice of adulterating and substituting the genuine herbal drugs are posing great obstacle in popularizing the time-tested herbal-based traditional medicines. The present study was focused on preliminary pharmacognostic and phytochemical investigations for determining and establishing the identity, purity and quality of the plants. The plant was subjected to determination of various physicochemical parameters including ash values (total ash, water soluble ash) and extractive values (alcohol soluble extractive, water soluble extractive). The powdered crude drug was extracted successively with various solvents with increasing polarity and further the extracts were subjected to phytochemical screening for the identification of various phytoconstituents. The study of heavy metal analysis, total bacterial count & test for Aflatoxins studies was also carried out as WHO guidelines. More over the fingerprint profile of Desmostachya bipinnata was established using thin layer chromatography(TLC) methods. The results of the TLC profiling of the extract confirm about the presence of various phytochemicals.