Antibacterial Activity of Chromatography Fractions from Jateorhiza macrantha (Hook F.) Exell Mendonça (Menispermaceae) Leaves
Igwe MN, Afieroho OE, Ugoeze KC, Abo KA
Page Numbers : 212-216
DOI : 10.36348/sjmps.2020.v06i02.011
Jateorhiza macrantha is a known medicinal plant used in ethno medicine for the treatment of diarrhea, cough, boils and ulcer. This study is aimed at evaluating the antibacterial activity of chromatography fractions from the aqueous ethanol extract of the leaves of Jateorhiza macrantha as a preliminary step towards isolation and characterization of its anti-bacterial constituent(s). The aqueous ethanol extract (AEE) was obtained by exhaustive cold maceration in 70% aqueous ethanol and further fractionated using vacuum liquid chromatography (VLC) techniques with a mobile phase gradient of n-hexane:chloroform:methanol (4:0:0, 3:1:0, 2:2:0, 1:3:0, 0:4:0, 0:3:1, 0:2:2, 0:1:3, and 0:0:4 v/v/v) into three fractions: non-polar (NPF1, eluted with n-hexane:chloroform:methanol 4:0:0, 3:1:0, 2:2:0 v/v/v), moderately polar (NPF2, eluted with n-hexane:chloroform:methanol 1:3:0, 0:4:0, 0:3:1, 0:2:2, 0:1:3 v/v/v) and polar (NPF3, eluted with n-hexane:chloroform:methanol 0:0:4 v/v/v). Antibacterial activity was evaluated using the agar well diffusion method against clinical isolates of pathogenic bacteria: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnuemoniae, and Salmonella typhi and the Minimum Inhibition concentration (MIC) extrapolated following the graphical approach. Functional group investigation was done using infra red spectroscopy. Three chromatography fractions NPF-1 –NPF3 obtained had MIC range of 5.0 mg/ml > MIC <0.26 mg/ml. NPF3 was relatively pure showing a single spot on TLC and its IR spectra are evidence for OH, N-H, C-O, C=C, and C-H stretching and deformation vibration frequencies typical of amino derivative of a lactone glycoside. The observed antibacterial activity justifies the ethno medicinal uses of Jateorhiza macrantha.
Original Research Article
Feb. 22, 2020
Comparison of Uncorrected Visual Acuity in Patients of Keratoconus Pre and Post Corneal Collagen Cross-Linkage with Riboflavin (C3R)
Dr. Rachana Gahlawat, Dr. Ashu Agarwal
Page Numbers : 206-211
DOI : 10.36348/sjmps.2020.v06i02.010
The present study comprised of 20 eyes of 14 patients with keratoconus presenting at a single centre. The study was conducted to compare the uncorrected visual acuity (UCVA) in patients before and after Corneal Collagen Crosslinking with Riboflavin (C3R). Follow- up period was 6 months. A comprehensive systemic and ocular history was taken and a through slit lamp examination, UCVA, refraction, fundus, intraocular pressure, pachymetry and corneal topography was done. Corneal collagen crosslinking was performed and UCVA was checked at 1 month, 3 months and 6 months. Statistical analysis was performed and it was noted that the mean UCVA improved statistically as compared to the preoperative value. Hence, UCVA is likely to improve in patients who undergo C3R.
Original Research Article
Feb. 20, 2020
Epidermomycosis at the Avicenne Military Hospital in Marrakech: 6-Year Review
Beddou G, SKALI H, El Mezouari E, Hocar O, Akhdari N, Amal S, Moutaj R
Page Numbers : 95-97
DOI : 10.36348/sjpm.2020.v05i02.010
Introduction: Epidermomycoses are superficial yeast infections that affect the epidermis outside the intertrigo. The aim of our work is to draw up a clinico-epidemiological profile of epidermomycoses diagnosed in the mycology parasitology laboratory of the Avicenne military hospital in Marrakech. Materials and methods: This is a retrospective study carried out over a period of 6 years (January 2013 to December 2018) including all the cases of epidermomycosis diagnosed. After collecting clinical information, a direct examination of the samples is carried out before culture on Sabouraud media with incubation at 27 ° C. The identification of the identity was based on the speed of growth, macroscopic aspects and microscopic colonies. The identification of yeasts was based on the filamentation and assimilation of sugars test: Api 20C. Results: Epidermomycosis was confirmed in 108 cases or 57% compared to all of the samples examined. The sex ratio M / F is 0.74, the average age is 35.5 years. Outpatients are the majority (86%). the prevalence of superficial dermatophyte mycoses was 81.4% followed by yeasts (14.6%) and molds (4%). Isolated dermatophytes are dominated by a single species Trichophyton rubrum (92%), yeasts by Candida albicans (45%) Conclusion: Epidermycosis is still a frequent reason for consultation in dermatology. In the face of any clinical suspicion, the mycological diagnosis remains the diagnosis of certainty and for therapeutic orientation.
Original Research Article
Feb. 19, 2020
Analysis of Flowrate in Loop Gas Pipeline Networks Using Pipe 2016 and Hardy Cross Method
OMOBOLANLE Oluwasegun Cornelious
Page Numbers : 49-62
DOI : 10.36348/sjet.2020.v05i02.003
Efficient and increased development of gaseous fuel as a complementary energy source requires a proper understanding of gas flow through pipeline networks and the required solution methods. This paper presents a comparative analysis of flowrate in loop gas pipeline systems using the Hardy Cross iterative method and Pipe 2016 (KYPIPE) computer program. A simple network model with four nodes, five pipe legs and two loops was manually analyzed based on the Hardy Cross procedure using Excel spreadsheet while comparing the effect of friction factors obtained from Moody’s chart, Colebrook’s implicit equation and Jain’s explicit equation on the result obtained from the Hardy Cross method. The accuracy of the result was validated by running a single period flow analysis on Pipe 2016 with the same network. Upon completion, a one-way analysis of variance (ANOVA) test was used to compare the four results while a T-test was used to double-check the three results obtained from the various friction factors based on Hardy Cross individually against the software’s result. Although, the mean plot showed that values obtained from Moody’s Chart and Colebrook’s equation compared more accurately with the software, the AVONA and T-test set at 5% significant level shows that no statistically significant difference exist between the results; making all friction factors suitable for use in gas pipeline flow analysis.
Original Research Article
Feb. 18, 2020
In the Name of Public Interest We Evict? (Re-actualizing the Standing Position of Proletarian Jurisprudence to the Marginalized)
Page Numbers : 21-32
DOI : 10.36348/sjhss.2020.v05i02.001
The phenomena of evictions of street vendors, homeless people, poor peasants, illegal settlers, etc. have been common in Indonesia. The weak proletariats are always sacrificed whenever they are vis a vis the bourgeois groups and rulers. If done continuously, the preservation of their religion, souls, intelligences, offspring, properties, and dignities will be menaced. So, re-actualizing fiqh mustaḍ’afīn (the proletarian jurisprudence) is urgently necessitated. This research calls for a re-conceptualisation of fiqh mustaḍ’afīn to present its importance and role in defending the rights of those oppressed group. The research was a mix-method type. Apart from studying related literature, it also employed fieldwork by interviewing a number of street vendors and government officers in Malang City, East Java, Indonesia. The results show that fiqh mustaḍ’afīn does belong to and favor those who are weak and oppressed and places those who do wrong and unjust as common enemies that must be fought.
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