Bullous lesions may be due to various causes, including bullous pemphigoid, pemphigus vulgaris, linear IgA disease, connective tissue disorders, and other rare vesico-bullous skin diseases. Though histopathology can help narrow down possible diagnoses, direct immunofluorescence is required for a definite diagnosis based on positivity patterns of different immunoglobulins. In this study, 47 cases of clinically suspected immune mediated vesiculo-bullous diseases (IMVBD) were confirmed with histopathology. Females were more commonly affected and the age range was from 10 to 93 years. Among these cases, 23 were diagnosed as pemphigus vulgaris, l6 as bullous pemphigoid, 3 each as pemphigus foliaceus and linear IgA disease. DIF, including testing for c1q, was instrumental for the diagnosis of the disease in order for the patient to get appropriate treatment.

Dengue and Zika viruses belong to the arbovirus family and are emerging infectious diseases transmitted by Aedes mosquitoes, prevalent in tropical and subtropical regions. Their proliferation is facilitated by factors such as adaptability, climate change, and globalization. Despite surveillance efforts by the World Health Organization (WHO), global monitoring remains limited. This study aimed to ascertain the seroprevalence of these diseases among travelers returning from endemic regions. A prospective, cross-sectional study was conducted on 2023 targeted travelers in the Democratic Republic of Congo and the Central African Republic. Participants were selected based on specific criteria over a six-month period. Blood samples were assessed in our laboratory using the VirClia® automated chemiluminescence immunoassay to detect Dengue and Zika IgG and IgM antibodies. The analysis involved interpretation of the results using a specific formula. The study included a young, asymptomatic cohort, averaging 30.8 years of age. Travelers were categorized according to age, sex, and country of residence. Dengue virus seroprevalence was 18.6%, predominantly IgG. The prevalence of ZIKV infection was 12.9%. The seroprevalence of these arboviruses varies according to sociodemographic factors. Epidemiological studies play a crucial role in assessing the prevalence of vector-borne diseases and informing public health strategies to mitigate their global impact.