Positive AnticyclicCitrullinatedPeptid Antibody in the antisynthetase syndrome should not lead to systematic diagnosis of association Antisynthetase syndrome-Rheumatoid arthritis. However their positivity is a marker of severity provider of destructive changes in joint damage in the antisynthetase syndrome without any diagnosis of Rheumatoid Arthritis established.

Invasive Streptococcus anginosus group (SAG) infection is associated with severe infection and poor outcome particularly among malignancy and post-surgical patients. We aimed to study the clinical details and outcomes of invasive SAG infection in post-sleeve surgery and malignancy related and non-malignancy related abdominal infections. (SAG) isolates from invasive infections between January 2015 and February 2018 were collected. Clinical data from the medical records of the infected patients were obtained retrospectively and analyzed. Fourteen invasive infections caused by SAG were identified. The mean age of the patients was 40.9 (minimum; 18 years, maximum; 80 years, SD; 21.03), five females (35.7 %) and nine (64.3 %) males. The peritoneal fluid was the most common specimen (8/15; 53.3%), followed by pleural fluid (3/15; 20%), deep abscesses (2/15; 13.3%), blood (1/15; 6.7%) and ascetic fluid (1/15; 6.7 %). The most predominant specimens (73.3%) were intra-abdominal involving commonly anaerobes and Enterobacteriaceae. Six patients (6/14; 42.9 %), had SAGabdominal or pleural infection as a complication of sleeve surgery. Each four of the remaining eight patients were categorized as patients with abdominal malignancy (4/14; 28.6 %) or non-malignancy related abdominal infections (4/14; 28.6%). Ten of the cases had polymicrobial infection. All patients had eventual recovery except of two malignancy patients who died because of severe sepsis with empyema or abdominal infection. Further studies are required to delineate post-operative sleeve surgery infectious complications and malignancy related abdominal infections to provide early treatment and better outcome.

The objective of the study was to evaluate the aphrodisiac property of the aqueous extract of Prunusafricana trunk bark in albino and normal male rats of the Wistar strain. Colorimetric and precipitation methods were used to highlight secondary metabolites of the plant. The acute toxicity test was conducted according to OCDE guideline 423. Four lots of 6 animals were used to evaluate the aphrodisiac property of P. africana. The positive control received sildenafil citrate (5 mg/Kg) and the negative control, distilled water. The test lots received the extract at 100 and 200 mg/Kg for 8 days. The copulatory parameters were observed on days 1, 4 and 8. The phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, saponosides and tri terpenes in the extract. No changes in the general appearance of the rats and no mortality were recorded during the toxicity test, highlighting that P. africana is nontoxic. The extract significantly increased the sex parameters of the tested rats. This aphrodisiac effect of P. africana attributed to identify secondary metabolites justifies its use in traditional medicine as a sexual stimulant.

Non-fermenters (NFs) account for about 15% of all bacterial pathogens isolated from a clinical microbiology laboratory. Primarily regarded as contaminants, they have been implicated in causing various infections. They are innately resistant to many antibiotics and emerging as important nosocomial pathogens. To isolate nonfermenters from Urinary Tract Infections and assess their clinical significance and antimicrobial sensitivity pattern.Five thousand nine hundred and thirty four (5934) nonrepetitive consecutive samples of urine from patients diagnosed of culture-proven UTI of a tertiary-care teaching hospital over a period of four-years (2014-2017) were selected. A urinary sample was subjected to presumptive screening followed by semiquantitative culture on CLED (Himedia, Mumbai) agar. Identification and antibiogram was done by Vitek 2 compact (bioMérieux, France) automated systems. Clinical history and laboratory parameters were utilized for clinical correlation.Out of 5934 samples showing significant growth 6212 (15.6%) bacterial isolates were grown, Non fermenters being in 5.6%. P. aeruginosa (55.17%)and Burkholderia cepacia (17.42%) were predominant NFs.

Ecotoxicity strength of different granular domestic detergents on Enterobacter aerogenes and Pseudomonas fluorescens was evaluated to assess the response of the test organisms to different brands of domestic detergents. Standard toxicity procedures were applied using four brands of granular domestic detergents: Klin, Omo, Bonux and Ariel prepared at concentrations of 10ppm, 100ppm, 1000ppm, 10000ppm and 100000ppm; the test was carried out for 0h, 4h, 8h, 12h, and 24h for each detergent.The degree of resistance of the test organisms to the toxicants revealed Enterobacter aerogenes (4857±1685ppm) was more resistant to the domestic detergents than Pseudomonas fluorescens (117±28ppm). Mean Median lethal concentration (LC50) showed that Klin was most toxic and Bonux was least toxic with toxic flow pattern (noting that the lower the LC50 the more toxic the toxicant) as:Klin (1354ppm) > Omo (2389ppm) > Ariel (2887.47ppm) > Bonux (3316.84ppm).Results of response of individual test organism to the different brands of detergents showed that Pseudomonas fluorescens was most sensitive to Bonux and least sensitive to Ariel - Bonux (94.86ppm) > Klin (104.66ppm) > Omo (109.68ppm) > Ariel (157.40ppm))while Enterobacter aerogenes was most sensitive to Klin and least sensitive to Bonux - Klin (2603.34ppm)> Omo (4668.68ppm) > Ariel (5617.54ppm) > Bonux (6538.82ppm). This showed that test bacteria responded differently to the toxic effect of the domestic detergents in the same aquatic environment. In mixed consortium, domestic detergent Klin had the highest toxic strength (mean LC50 1354ppm); Pseudomonas fluorescens was more sensitive to all the test domestic detergents than Enterobacter aerogenes.

Kidney diseases rank 3rd amongst life threatening disease, after cancer and heart disease. Anemia is an constant complication of chronic kidney disease that significantly contributes to the symtoms and complications of the disease. Aim of the study is to study the hematological manifestations ofchronic kidney disease (CKD) and to correlate the hematological abnormality with the clinical stage.182 patients with chronic kidney disease admitted in Osmania general Hospital were included in the study for a period of 2 years. Patients in various stages of the disease were studied for various hematological parameters. CKD was seen across all age groups. Increased prevalence of CKD was seen in the age group of 41 - 50 years. Majority of patients at presentation were in stage V CKD. Hypertension (47.2%) was the leading cause of CKD, followed by diabetes. Ischemic heart disease was the most common associated illness seen in CKD. The mean hemoglobin in the present study was 7.99 +/- 1.2 g/dl. The fall in mean hemoglobin had an inverse correlation with the stage of CKD. The mean RBC count was 2.73 x1012/l, with significant fall in the RBC count as the stage of CKD progressed. The mean absolute reticulocyte count also showed significant decline as the stage progressed.Chronic kidney disease is seen across all age groups with a malepreponderance. Hypertension is the most common cause of CKD. The anemia of CKD is a normocytic normochromic anemia. The fall in hemoglobin is due to low RBC count due to decreased erythropoiesis.

Primary actinomycotic infection is a rare finding in breast with very few cases been reported in male breast and it is seen to be associated with trauma to skin or any predisposing conditions like diabetes mellitus. We are reporting an unusual rare case of actinomycosis of breast in a 70 year old male patient who came with the complaints of swelling in the right breast since seven years along with pain and bloody discharge from nipple since five to six months. Clinically breast malignancy was suspected and lumpectomy was done. Histopathological study showed features of actinomycosis.

In Morocco, the number of people aged 60 and over has increased. This draws attention to conditions whose prevalence increases with age including chronic kidney failure and dementia. This population appears to have a higher risk of cognitive impairment. The aim of our study is to determine the prevalence of cognitive disorders in our dialyzed patients, to define the types of this decline and identify the factors associated with cognitive decline. Observational, cross-sectional study of 116 patients taking hemodialysis from El Ghassani hemodialysis center in Fez. We used the Montreal cognitive assessement test before the hemodialysis session, A Moca ≤ 24/30 indicate the presence of cognitive impairment. The Moca was abnormal in 70% of our patients. The average score was lower for women. The areas most affected were attention and short-term memory. We found a strong correlation between the decrease in the Moca score and the progression in age (p <0.001). Hypertension was correlated with a Moca score with an average score in the hypertensive population of 20 ± 5.4 (p <0.006). The relationship between cardiac disease and Moca was significant with an average score in patients with heart disease of 19 (p <0.01). Taking an erythropoiesisstimulating agents were linked to the Moca test (p <0.05). The number of sessions per week was significantly correlated with Moca (r: 0.25, p <0.005). Albumin and hemoglobinemia were correlated with the Moca score (r: 0.2, p <0.027) and (r: 0.2, p <0.02) respectively.

Dengue is a viral infection that affects millions of lives each year. A rapid screening test that would detect the NS1 antigen, IgM and IgG antibodies to recombinant antigens of all the dengue viruses helps in early detection of a potentially fatal disease. SD Bioline Duo, with the lateral flow technique, was employed in 24 cases of suspected dengue fever over a one-month period in a tertiary care hospital, with 14 cases (58.33%) testing positive. It helped in timely management of the cases and prevention of further complications of the viral disease.

The Aims and objectives of the study are toprovide a standard for good laboratory practice, to increase the clinical effectiveness, patient safety and operational efficiency and designing better and more evidence-based systems for the timely notification of laboratory results which represent potentially hazardous to the patient.This concept was introduced 46 years ago by Lundberg. It is a result, indicating that patient is in imminent danger unless therapy is initiated immediately. A prospective cross-sectional study was done at ASRAMS, a 1000 bedded hospital at Eluru, from July 2017 to June 2018 for a period of 12 months. The laboratory performed 2,22,574 tests in haematology and 2,92,055 tests in clinical pathology. The preparation and approval of critical alert value list was done in consultation with our hospital board and clinician’s panel. During a one year period of study, a total of 7148 critical values were reported. 45.32% of critical alert values belong to inpatient, 48.9% to outpatient and 5.8% to emergency department.We finally Conclude that it is crucial for patient safety, Effective use of the available resources, Creates professional responsibility and Regular quality assurance, meeting with technical staff and strict vigilance are key reasons for low figures in our setting.

Computer keyboards can contribute to the spread of microorganisms, mainly when shared by multiple users. Evaluate bacterial and fungal contamination in shared computers keyboards used in a University and propose preventing measures. This research has an investigative, descriptive and transversal design. Keyboards from 60 computers of collective use were randomly selected. Surface samples were collected with sterile swabs and moistened in 0.9% NaCl solution. The swabs were rubbed against the surface of the keyboards and then stored in Stuart's transport medium and sent to the Bacteriology Research Laboratory of the Souza Marques Medicine School. The material was seeded in the culture medias blood agar, hypertonic-mannitol-agar, Sabouraud-dextrose-agar and Micosel. Bacterial colonies were identified through morphotinorial characters, biological and biochemical tests. Yeasturiform fungi were identified by biochemical tests and the filamentous fungi through morphotinorial and cultural characters. From the 60 examined samples, 10 different bacteria species were isolated: Bacillusspp 18 (30%), Enterococcusspp 5 (8.33%), Streptococcus alfahaemolyticus 8 (13.33%), Escherichia coli 4 (67%), Klebsiellaspp 2 (3.33%), Enterobacterspp 6 (10%), Proteusspp 1 (1.67%), Neisseria spp 3 (4%), Staphylococcus aureus 5 (8.33%), Coagulase negative Staphylococcus 32 (53.3%). Isolated fungi: Candida albicans 3 (5%), Penicilliumspp 15 (25%), Aspergillusniger 8 (13,33%), Aspergillusfumigatus 2 (3,33%), Alternariaspp 4 (6,67%), Epidermophytonfloccosum 2 (3.33%), Fusariumspp 7 (11.67%), Rhodotorulaspp 10 (16.67%). The keyboards of the shared computers were contaminated with fungal and bacterial elements, and among them E.coli, evidence of faecal contamination. This contamination occurs due to the high turnover of users with different hygiene habits. Thus, a hygiene protocol is required to minimize contamination of computer’s keyboards.