Transfusion of Fresh frozen plasma is relatively common in neonatal intensive care units. Most common indications for transfusion being neonatal sepsis, deranged coagulation profile or co existence of both these conditions. However a universal protocol for FFP transfusion in neonates is lacking, leading to injudicious administration of FFP to neonates in many hospitals and thus exposing them to untoward risks of transfusion. This is our attempt towards analyzing the use of FFP in our neonatal intensive care unit and our compliance with the established guidelines (AIIMS protocol for FFP transfusion in neonates). Data was collected retrospectively of all the neonates who received FFP transfusions in our hospital from January 2014 to December 2017 and relevant analysis was performed. Most of the neonatal transfusions (93%) at our hospital during the study period were compliant with AIIMS guidelines. Most common indication for transfusion was bleeding manifestations followed by sepsis and deranged coagulation profile. However on analysis of pre and post transfusion coagulation parameters in these babies, no statistically significant improvement was seen. FFP was often used in accordance with the published guidelines in our study. However evidence based uniform guidelines need to be established and implemented to increase the benefit and minimise the risk associated with transfusion, especially in newborns.

Prostate gland of male reproductive system is about the size of walnut and surrounds the urethra. With increasing life expectancy, increasing awareness and better health services, lesions of prostate has become a common specimen received for diagnostic of both benign and malignant lesions which may have a very similar presentation but their management and prognosis is quite different. Most important investigation is the biopsy of the prostate. A histopathological study of features of the lesion gives best diagnosis. Our objective of study is to evaluate the spectrum and correlation of prostatic lesions with presenting complaints of the patient. It was a combined cross-sectional study conducted in Pathology Departments of both Bhaskar Medical College and Satya Diagnostic Centre, Himayath Nagar during the period from November 2015 to October 2017.

Endoscopy with endoscopic biopsy is currently the major method of diagnosis of gastrointestinal (GI) neoplasms. A total of 60 endoscopic biopsy specimens from upper and lower gastrointestinal tract were studied in the laboratory from November 2013 to October 2014 in the department of pathology, Acharya Shree Chander College of Medical Sciences and Hospital (ASCOMS), Jammu. Of these gastrointestinal biopsies 25 (41.66%) were from upper gastrointestinal tract and 35 (58.33%) were from the lower gastrointestinal tract. Among these upper and lower GI biopsies a total of 35 (58.33%) suspected neoplastic lesions were included in the present study. The biopsies that were included in the study comprised of 6 (10%) esophageal biopsies, 12 (20 %) gastric biopsies, 3 (5%) from GEJ, 4 (6.66%) from duodenum, 2(3.33%) from pyloro-duodenum, 1 (1.66%) from jejunum, 2 (3.33%) from ileum, 14 (23.24%) from colon, 12 (20%) from rectum, 4 (15%) from anus. There are only few studies on histopathological spectrum on GI endoscopic biopsies in Jammu region. ASCOMS is a tertiary care hospital where patients come from all over Jammu region. Analysis of biopsied material therefore can provide a fairly good estimate of spectrum of various gastrointestinal lesions (neoplastic/non-neoplastic/pre-neoplastic) in Jammu region as well as comparison of our results with similar studies in other institutions.

Visceral Leishmaniasis is a vector-borne parasitic infection caused by a parasite of the genus Leishmania. This disease, which is more frequent between children than adults, knew during these last few years in Morocco a significant surge especially in the region of Fez. The direct examination, based on the detection of the parasite or its nucleic acid, is undoubtedly the key to a definite diagnosis, along with serological techniques that were proven to be efficient and could also aid the diagnosis. This study highlights the benefit of rK39 rapid tests in visceral Leishmaniasis diagnosis through the retrospective of 75 patients suspected to have Visceral Leishmaniasis. We shall also compare our results with those of other authors in different countries.

Pseudomonas aeruginosa is a true opportunistic pathogen and is responsible for causing a variety of infections in clinical settings in both immunocompetent as well as immunocompromised hosts. However, in most cases, P.aeruginosa infections occur in patients who have been compromised in some way, e.g. chronic Diabetic Patients. Methods: In this study a total of 280 (10.98%) P.aeruginosa isolates were obtained out of 2549 samples collected for a period of 5 years. The isolates were selected on the basis of their growth characteristics on Blood agar, MacConkey agar and Nutrient agar medium. Colonies were subjected to series of biochemical tests to identify the species. Antimicrobial susceptibility testing of all the confirmed P.aeruginosa isolates was performed by automated walkaway 96 using NBC 42 and conventionally comparing with Kirby–Bauer disc diffusion method for further confirmation and results were interpreted according to CLSIs guidelines. Results: The prevalence of this pathogen was 10.98% and most of the isolates were mostly isolated from pus/wound swab cultures, followed by urine & sputum cultures, mostly isolated from critical areas such as ICU’s.

Tuberculosis, inspite of advances in diagnosis and therapeutics still continues to be the leading cause of mortality and morbidity in many developing countries even today. It is one of the major killer infectious disease. In recent years, two important factors which heavily complicate the treatment outcome of tuberculosis patients are MDR-TB and XDR-TB.The mortality in Tuberculosis mainly due to delay in diagnosis. India has the highest TB burden accounting for 1/5 th of Global incidence. The most common form of TB is pulmonary tuberculosis. The sputum microscopy and culture both of them are backbone for the diagnosis of Tuberculosis. The present study aimed to compare and evaluate the of Trisodium Phosphate solution as sputum concentration technique by smear microscopy (Zeihl Neelsen Technique), to compare the smear microscopy. Sputum samples of suspected 150 cases of pulmonary tuberculosis attending Thoracic Medicine and General Medicine OPD at Thanjavur Medical College, (Tamil Nadu) were collected and processed for smear microcopy. Among the 150 sputum samples, AFB positivity in unconcentrated sputum microscopy by Zeihl Neelsen Technique-33(22%),AFB positivity in concentrated sputum microscopy by Zeihl Neelsen Technique-37(25%).The present study confirms that the importance of Trisodium Phosphate solution for sputum concentration technique for the smear microscopy by Zeihl Neelsen Technique.

Mesenchymal hamartoma of liver is the second most common benign liver tumor in children after hepatoblastoma. First described in 2006 as multicystic biliary hamartoma, it generally presents as large multicystic mass in less than three years of age.We report one such case in a 3 year old male child who came with history of fever and rash and was incidentally diagnosed with a cystic mass in the liver. Partial hepatectomy was done which on microscopy revealed epithelial structures in loose connective tissue stroma along with cystically dilated spaces lined by columnar and flattened epithelial cells, typical of Mesenchymal Hamartoma of liver. We are presenting this case because of its rarity.

β-Lactam antibiotics are commonly used to treat bacterial infections. Increased use of antibiotics, particularly the third generation of cephalosporins, has been associated with the emergence of ESBL producing bacteria, most commonly in E. coli. And Klebsiella pneumonia. Infection due to ESBL producers range from uncomplicated urinary tract infection to life threatening sepsis. This study was undertaken to assess the prevalence of ESBL producing E.coli and Klebsiella isolates. Patients taking treatment in P.D.U. Hospital and Medical College, Rajkot- a tertiary care hospital in Gujarat, India, were included in study. During the half year period from July 2014 to December 2014, a total of 600 E. coli and Klebsiella isolates from various clinical specimens were included in the study. The isolated organisms of E. coli and Klebsiella were characterized for their production of ESBL. Out of 600 E. coli and Klebsiella isolate, E. coli were 224 (37.3% and Klebsiella spp. 376 (62.7%). The overall rate of production of ESBL (by phenotypic confirmatory test with double disc diffusion method) in total isolates of E. coli and Klebsiella spp. is 22.5% (135/600). The rate of production of ESBL in E. coli is 29.9% (67/224) and in Klebsiella spp. is 18.1% (68/376). ESBL producing E. coli and Klebsiella showed high prevalence in present study. Routine laboratory testing for ESBL is needed in order to optimize antibiotic management to reduce ESBL associated morbidity & mortality.