A blood film or peripheral blood smear is a thin layer of blood smeared on a microscope slide and then stained in such a way to allow the various blood cells to be examined microscopically. This can be done by manual method or with automated analysers. This study is based on comparative study of manual method and automation (SC 120 auto slide maker and stainer) in making of blood films for evaluation.

Acute megakaryoblastic leukemia, or type 7 (AML 7), is a rare entity defined by a proliferation of blasts of which at least 50% are megakaryoblasts. It represents 0.5 to 2% of acute leukemias (LA) in children and 3 to 10% of acute myeloid leukemias (AML). Patients may have nonspecific symptoms. His diagnosis is based on the presence in the blood or in the marrow of blasts with typical morphology and expressing specific platelet antigens. This observation describes the clinical case of a 19-month-old infant with pancytopenia. The diagnosis of AML7 was made based on explorations of peripheral blood, myelogram, and immunophenotypic analysis. Chemotherapy has been started, with a poor therapeutic response. The patient died in a state of septic shock. We recall through a literature review the clinical, histological and immunophenotypic features of acute megakaryoblastic leukemia.

In developing countries, the sexually transmitted infection is one of top five diseases to which adults seek for medical help. There is an association between common STIs and sexual transmission of HIV. Therefore, control of STIs receives attention to control the spread of HIV. A retrospective study was conducted in Teaching Hospital Batticaloa during the period between 2014 and 2016. A total of 1190 cases were newly registered in STD clinic, of which 317 cases were confirmed to be having STIs. A total of 317 confirmed cases of infection episodes were recorded in all patients attended to STD clinics during this three years. Of which, 96.8% were due to STI and 3.2% due to lower reproductive infections. Among the STIs; genital herpes (22.71; Syphilis (16.71%). gonorrhoea infection 4.41%. Trichomonas vaginalis was 7.25%. During the period of three years only one case of HIV was identified. Hepatitis B infection constitutes to 15.14% stays in order next to Syphilis .bacterial infections shows a decreasing trend while a gradual increase of viral infections is observed. Public health should be strengthened especially use of condom and avoiding sexual contact with unreliable sexual partner in order to control STIs

We present a rare entity of incidentally detected microscopic thymoma in the thymectomy specimen of a female with early onset myasthenia gravis. The thymus was of normal size radiologically and macroscopically. Histological sections examined show multifocal tiny thymic epithelial proliferation suggestive of microscopic thymoma. Microscopic thymomas are rare tumours and less than 20 cases have been reported worldwide

The skin is the largest organ in the body. A multitude of diseases are linked to an insufficient or overactive dermal vasculature. Vessels change their phenotype,i.e. vascular remodelling in inflammatory conditions. The aim of this study was to analyse the dermal vascular alterations in various inflammatory skin diseases & to assess their utility in establishing the differential diagnosis. A unicenter study of 55 (fifty five) skin biopsies received from patients of various non tumorous common inflammatory skin diseases such as, psoriasis, lichen planus, erythema multiform, blistering (bullous) lesions and 10 control samples were studied. Morphometric analysis was performed on CD 34 immunostained sections using image analysis software (Leica Q Win V3 software analysis system). Mean vascular density (MVD) and mean vascular calibre (MVC) was calculated. The MVD was maximum in psoriasis, followed by lichen planus, bullous lesions and erythema multiform. MVD in psoriasis was significantly higher than the controls. MVC was highest in psoriasis followed by erythema multiform, lichen planus and bullous lesions. The MVC in psoriasis, erythema multiform, lichen planus, bullous lesions were significantly higher than the controls. Elongated, tortuous blood vessels were more prominent in psoriasis. The endothelial changes noted were swelling in the form of nuclear hypertrophy, increased cytoplasm and endothelial gaps. Angiogenesis was increased in inflammatory skin diseases and it was highest in psoriasis. Therefore, therapeutic intervention at the level of vasoproliferation may prove to be useful.

This study was carried out to evaluate the antimicrobial activity of hexane, chloroform, ethyl acetate and water extracts of six marine macroalgae belonging to different families namely green algae (Chlorophyceae), brown algae (Phaeophyceae) and the red algae (Rhodophyceae) collected from the intertidal area of the Sudanese Red Sea coast near Port Sudan. Successive extraction techniques using hexane, chloroform, ethyl acetate and water were used for extracting the active principles of the algae and the disc-diffusion method was performed to examine the activity of the crude extracts against four pathogenic bacteria and one fungus. Most of the tested algal extracts exhibited considerable bioactivity and inhibited the growth of most of pathogenic microorganisms under investigation. The chloroform extract of the red alga Gracillaria sp produced a maximum inhibition zone of 22 mm against Escherichia coli whereas the chloroform extract of the red alga Laurencia papillosa showed a low antimicrobial activity with a minimum inhibition zone of 5 mm against Bacillus subtilis.