Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CLA) in small ruminants. This study was designed to evaluate the efficacy of 0.5% and 1% formalin killed prototype vaccine of C. pseudotuberculosis in bucks and does. A total of 24 healthy bucks and does were equally divided into four groups of 6 animals. Groups A and B were vaccinated with 0.5% and 1% formalin killed prototype vaccine and groups C and D were kept as positive (+) and negative (-) control groups, inoculated with plain vaccine adjuvant. After booster vaccination at the 6th week, groups A, B and C were experimentally inoculated with 2mL of 106 cfu of live C. pseudotuberculosis subcutaneously. On the 12th week, all animals were euthanized and samples of reproductive organs and pituitary glands of both bucks and does were collected. Bacterial isolation and identification was done on the basis of morphological characteristics and PCR detection. Based on the bacterial culture results, 3 samples each were Positive for C. pseudotuberculosis colonies from bucks in groups A and B, while 4 samples were positive from does in group A and only 1 from does in group B. All bucks and does from groups A and B showed negative PCR amplifications in all samples. This study showed that 1% formalin killed prototype vaccine of C. pseudotuberculosis is effective in preventing the spread of the bacteria to the reproductive organs and pituitary glands of both bucks and does. Thus, it will be a suitable preventive measure against CLA in small ruminants.

The occurrence of monoclonal gammopathy of undeterminate significance (MGUS) in an autoimmune disease such as systemic lupus is not common but it is not as rare as the occurrence of a malignant disease such as Multiple myeloma. The complexity of this association lies in the challenges of monitoring MGUS’s patients who are likely to progress towards malignant hemopathy. Some risk factors for this progression have been described (rate of monoclonal compound, paraprotein isotype, medullary plasmacytosis or even light chains). However, none of these factors taken independently allows us to correctly categorize patients. Scores combining several of these factors have been proposed to define groups of patients with different evolving risks. The aim of this work is to report this association (systemic lupus and MGUS) in a 52 year old woman, in order to alert the clinicians about this eventuality when monitoring their lupus patients.

Surface epithelial tumors of the ovary account for approximately two-thirds of all ovarian neoplasms and their malignant forms represent about 90% of ovarian cancers. For years, efforts to identify reliable prognostic factors have focused on molecular markers; large number of them has been investigated to date, usually by immunohistochemistry. Mutation of the p53 gene has been reported in a variety of human malignant tumors and it has been claimed to be a marker of poor prognosis. Aim of the study is to assess the immunoexpression of p53 and Ki 67 in surface epithelial tumors of the ovary and to evaluate their correlation with the clinicopathological parameters. There were 60 cases of surface epithelial tumors encountered over the period of 7 years from 2007 to 2014 in addition to10 samples of nonneoplastic ovarian tissues were included as a control. The samples were collected from Hawler Maternity Teaching Hospital. Formalin fixed, paraffin-embedded specimens were studied and sections were prepared for immunohistochemical staining for both p53 and Ki 67 markers. Ethical consent has been obtained from Hawler Medical University. Data were analyzed using the Statistical Package for Social Sciences (SPSS, version 19). High immunoexpression of p53 and Ki 67 were seen in patients with ovarian tumor aged more than 40 years. Statically highly significant correlation was found in the immunoexpression of both p53 and Ki 67 marker between benign, borderline and malignant surface epithelial tumors ( P value < 0.05); while no significant correlation was found between their expression in relation to tumor grade and stage in epithelial ovarian cancer. Maximal immunoexpression of p53 and Ki-67 immunohistochemical markers was seen in epithelial ovarian cancer, this emphasizes their important carcinogenic role in surface epithelial tumor of the ovary. Their over expressions are not correlated with tumor grade and stage.

Dengue infection is a serious global public health problem, and the dengue viruses are widely distributed throughout the tropical and subtropical areas of the world. In the Indian subcontinent, the epidemiology of dengue fever has been very complex. High prevalence rate in our region particularly in pre-monsoon and monsoon season is alarming and necessitates early and accurate diagnosis of dengue virus infection to prevent its complications and further morbidity. Now-a-days detection by rapid tests offers an even faster route to a presumptive dengue diagnosis. Hence, this study aims to assess the agreement between the rapid immune-chromatographic card test (RICT) for dengue and antibody capture ELISA, the latter being used as gold standard. A prospective study was conducted from July to November 2014 and July to November 2015 in a secondary care hospital. Probable dengue cases were diagnosed as per the WHO criteria and RICT and ELISA were conducted on the same serum samples for the detection of NS1 antigen and IgM & IgG antibodies. The results were analyzed. The sensitivities of RICT for NS1antigen and IgM & IgG antibodies were 100%, 89.6% and 81.3%, respectively and all showed 100% specificity. In the present study, the accuracy parameters were comparable with ELISA. Also, RICT met the WHO ASSURED criteria, hence this study recommends introduction of RICT in all levels of health care system for an early and accurate diagnosis and confirmation of the clinical suspicion of dengue.

Prolymphocytic leukemia T (PLL-T) is a rare entity of mature lymphoproliferative syndrome, characterized by its evolution but especially by its poor prognosis. It is usually revealed by hyperlymphocytosis in the blood. We report a case of prolymphocytic leukemia T, revealed by blood hyperlymphocytosis higher than 800 G/L, diagnosed at the My Ismail military hospital of Meknès, Morocco.

Serum malondialdehyde (MDA), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and histological analysis of lungs were carried out in twenty rats exposed to smoke from transfluthrin impregnated insecticide paper. The rats were divided into five groups (I, II, III, IV and V) of four each. Group one served as control while Group II, III, IV and V were exposed to smoke from transfluthrin impregnated paper Insecticide for 15, 30, 60, 120 minutes daily for four weeks respectively. On the 29th day, the rats were euthanized and blood sample was collected and centrifuged for analysis of biochemical parameters (MDA, AST, ALT and ALP), the animals were dissected and lung tissues were collected for histological analysis. Significant increase (p<0.05) in all parameters (MDA, AST, ALT and ALP) were observed in a time dependent pattern compared to normal control. However, histopathological analysis of the lung tissues shows no pathological changes between test groups and normal control. Thus, exposure of rat to smoke from transfluthrin impregnated paper insecticide modifies biochemical parameters (MDA, AST, ALT and ALP) but within the context of duration of this research, no significant pathology was observed in lung tissues. Transfluthrin impregnated paper insecticide should be used with caution.

Colorectal diseases are frequently encountered in clinical practice. They range from minor problems causing minimal discomfort to potentially serious diseases causing much morbidity and mortality. Clinically most of these disorders present with non-specific symptoms like altered bowel habits, diarrhea, bleeding per rectum either melaena or fresh hemorrhage; thus causing much delay in drawing attention. Early detection and proper management are essential to reduce the morbidity and mortality. Various diagnostic tools are developed in the recent past, improving the detection and assessment of these lesions. To study the profile of various neoplastic colorectal lesions and to know the relative frequency of various diseases, and to know the age, sex incidence and site of occurrence of the neoplastic colorectal lesions. This study was carried out in the Department of Pathology, NRI Medical College, Chinakakani, Guntur district.

Hypereosinophilia is defined by blood eosinophil count greater than 0.5 x 109 / L, it is a biological symptom which should not be neglected given the risk of visceral complications. However, any eosinophilia should be thoroughly investigated in order to define the etiology and treat it. Diagnosis of idiopathic hypereosinophilic syndrome HES can be retained after elimination of parasitic, allergic and haematological causes in the presence of a blood eosinophilia greater than 1.5 x 109 / L evolving over a period of at least six months and complicated by at least one visceral lesion. The visceral sites observed are not specific among which the cardiac and neurological attacks are the most dangerous compared to the other attacks: bronchopulmonary, liver, digestive, renal, cutaneous. We report an observation of persistent hypereosinophilia in a patient with exclusively digestive signs and the diagnostic difficulties encountered.