This study dealt with the assessment of the phytochemicals, antibacterial and mineral analysis of methanol extract of leaf Newbouldia laevis and its fractions (hexane, chloroform and ethyl acetate). Quantitative phytochemical screenings were done by various standard laboratory methods. Quantitative phytochemical screening was carried out using spectrophotometry method while mineral analysis was determined by atomic absorption spectrophotometric method. Antibacterial activity was investigated using agar well diffusion method. The result obtained from the phytochemical screening revealed the presence of alkaloids, phenols, oxalate, tannin, saponin among others. The antibacterial activity of Newbouldia laevis leaf extracts was tested on Escherichia coli, Staphylococcus aureus, Klebsiellia pneumonia, and Streptococcus pneumonia. Antibacterial effect of Newbouldia laevis leaf showed that only nhexane fraction was capable of inhibiting all microorganisms used except Staphylococcus aureus in concentration dependent manner. Chloroform fraction was found to inhibit only E.coli at higher concentration (100-150 mg/ml) as indicated in the zone of inhibition, while it has no effect on other bacteria. Methanol extract showed inhibition activity only on S. aureus at 100-150 mg/ml. All the microorganisms showed resistance to the aqueous fraction of Newbouldia laevis leaf. Mineral analysis revealed the presence of Zn, Co, Cd, Cu, Ca, Fe and Mn. These results could suggest the promising chemopreventive use of Newbouldia laevis and some of its active principles in the treatment of infections.

Catheter associate Urinary tract infection (CAUTI) is common health care–associated infection and widely found in hospitals and long term care facilities. Recent studies reported urinary pathogens showing resistance to most commonly prescribed antibiotics. Area specific study to find out the pathogen responsible for CAUTI and their resistant pattern which will be helpful for clinician to choose proper treatment and strategies to prevent infection in future. The infection rate was 52% among 200 patients using indwelling catheter. It was anticipated when catheter was used for longer period infection rate up to 60%. E. coli was most frequent detected bacteria and amikacin found most effective among the antibiotic we tested. Overall hospital stay and financial burden of patients increased due to CAUTI compared to the patients without infection.

Visceral leishmaniasis may be considered as a rare opportunistic infection in adult with chronic lymphocytic leukemia. We report the second case in the world and the first one in Morocco. It is about a 70-year-old patient, with chronic lymphocytic leukemia treated with chemotherapy. He presented fever, splenomegaly and axillary adenopathy. The blood count showed persistent pancytopenia. The bone marrow aspiration showed the presence of Leishman’s bodies. The patient was lost to follow-up.

This is the first study of aeromycoflora from the two places Ad Darb and Shuqaiq in the Jizan province. The diversity of aeromycoflora of the two places was isolated to know the environmental status. A total number of sixteen outdoor and indoor environmental samples of aeromycoflora were collected and studied from the two places along with the College of Science and Arts for Girls at Ad Darb. The aeromycoflora was cultured by open petri plate method during the month of April 2017 . A qualitative and quantitative assessment of the indoor and outdoor mycoflora was carried out by exposing the Potato Dextrose Agar petri plates in the different environments each for a period of two minutes.The fungal cultures on the petri plates and the slides were identified by microscopic and macroscopic characteristics. The outdoor aeromycoflora was represented by Aspergillus niger, Aspergillus flavus, Aureobasidium, Curvularia, Dresclera, Epicoccum, Fusarium, Penicillium and Yeast. The indoor aeromycoflora was represented by Aspergillus, Colletotrichum , Penicillium, Chrysosporium and Ulocladium. The most predominant genus was Aspergillus. The colony forming unit (CFU)3 of each of the represented mycoflora was calculated to find out the level of contamination at the different sites. The (CFU)3 values indicated that the outdoor mycoflora was higher than the indoor mycoflora .The fungal genera isolated are pathogenic. Hence a polluted aerial environment is harmful not only to human health but also to the animals and plant life .Therefore a constant monitoring for a clean and germ free environment is needed at all times.