Synthesis and investigation of new Co(II), Ni(II), Cu(II), Zn(II) and Cr(III)chelates of mixed ligands including aSchiff base [(E)-2-(4-(dimethylamino)benzylideneamino)phenol] (C15H16N2O) derived from the condensation of 4- dimethylaminobenzaldehyde with 2-aminophenol as main ligand (L1) and an amino acid; L-Histidine as co-ligand (L2) were studied. The obtained Schiff base and the mixed ligand chelates were subjected to several physiochemical techniques, in terms of CHN elemental analyses, molar conductivity, magnetic moment measurements, infrared, electronic and mass spectroscopies. The CHN analytical data showed the formation of the Schiff base compound and the mixed ligand chelates in 1:1:1[M:L1:L2] ratio. All the prepared mixed ligand chelates were non-electrolyte in nature. The infrared spectral data exhibited that the used ligands behaving as bidentate ligands towards the metal ions. The 1HNMR spectra of the ligands and their Zn(II) mixed ligand chelate exhibited the effect of the activated groups of the ligands by the metal ion. The electronic spectral results showed the existence of π→π* (phenyl ring) and n→π*(HC=N) and suggested the geometrical structures of the chelates. Meanwhile, the mass spectral data revealed the fragmentations of the Schiff base, Histidine and their Cu(II) mixed ligand chelate. The studies made on these chelates proposed a six coordinated octahedral geometry for all these chelates. The antibacterial activities of the Schiff base, Histidine, metal salts and mixed ligand chelates were screened. It is found that the mixed ligand chelates have the most biological activity in comparison to the free ligands and salts.

The current work reports a new Schiff base [N1-benzylidenebenezene-1,2-diamine(L) = C20H16N2] has been synthesized from benzaldehyde (C6H5CHO) and O- aminoaniline (O-C6H4(NH2)2. Metal mixed ligand complexes of the Schiff base were prepared from chloride salts of Zn(II), Cd(II) and Hg(II) in ethanol and 8- hydroxyquinoline(8HQ)(C9H7NO) containing sodium hydroxide. All the complexes were characterized on the basis of their; FT-IR and U.V spectra, melting point, molar conductance, and determination of the percentage of the metal in the complexes by flame (AAS). In the all complexes, (8HQ) behaves as a bidentate ligand as primary ligand through –-OH phenolic group and –N groups of pyridine group. Also, the prepared ligand (L) was bidentate in all complexes coordinating through two nitrogen atoms as a secondary ligand. Mixed ligand complexes of bivalent ions of Zn(II), Cd(II) and Hg(II) of the according to the formula [M(L)( Q)2] in 1:1:2 of molar ratio. The suggest geometry of the complexes appears to be octahedral. The synthesis complexes were tested in vitro for antibacterial activity of ligands and metal complexes to the pathogenic bacteria activity against bacteria (gram +ve) and (gram -ve) has been studied.

Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus fermentum isolated from fermented foods: yoghurt, “ugba” and “kunu-zaki” using MRS agar and a commercially acquired lactic acid bacteria (LAB) were used to produce yoghurts samples A-H from Milksi Powered milk in a 5 hour fermentation process. The three LAB isolates were used singly and in combinations as starter cultures. There was a maximum drop in pH (4-4.8) between the second and third hour of fermentation while the yoghurt produced using S. thermophilus had the lowest pH (5.2) at the temperature of 40oC. The optimum pH for the yoghurt production was 5.5 while the optimum temperature was 40oC. Yoghurt samples B and F had the highest moisture level (P<0.05) while Samples C, E and G had the highest dry matter content. The highest ash content (0.8 ±0.01; P<0.05) was from sample F while Samples D and E had the highest crude fibre content. Sample A had the highest crude fat value (3.95a±0.01; P<0.05), Sample F the highest crude protein content (3.95a±0.01; P<0.05) while the highest carbohydrate content was from Samples C, E, G and H (control). The control Sample (H) had the highest and Hedonic test for sensory properties of the eight yoghurt samples showed that the eight yoghurt samples were acceptable to the panelist indicating that the samples would compete favourably in the market with commercially sold yoghurts.

Swellings of the major salivary gland commonly lead to the differential diagnosis which includes benign and malignant tumors that are frequently associated with cystic degeneration or entirely cystic de novo. Only 6-9% of major salivary gland swellings are cystic lesions and 2-5 % are non-neoplastic in nature. This is a case report of such an asymptomatic unilateral parotid gland lesion in a 30-year-old female patient. The authors discuss the significance of clinical, radiographic, macro and microscopic correlations of cystic mass in major salivary glands.

Hysterectomy is the most commonly performed major gynecological surgery worldwide and prevalence of hysterectomy varies from country to country, region to region. Hysterectomy may be total -removing the body, fundus, and cervix of the uterus; often called complete hysterectomy or partial hysterectomy/subtotal hysterectomy - removal of the uterine body while leaving the cervix intact; also called supracervical hysterectomy. The objective of this study is to know the most common pathology causing abnormal uterine bleeding by studying histopathological findings in hysterectomy specimens and also to know the commonest age group undergoing hysterectomies. In our study involving 200 cases most common age group underwent hysterectomy was 40-49 years followed by 30-39 years group and least hysterectomies done in group 70-79 years. Type of hysterectomy performed most commonly in this study was supracervical or subtotal hysterectomy. Most of lesions were seen in the endometrium 84 cases (42%), Myometrium 59 cases (29.5%), cervix 33 cases (16.5%) and ovary 24 cases (12%). Hysterectomy still remains the widely used treatment modality in developed and developing countries. All hysterectomy specimens should be sent for histopathological examination regardless of the pre-operative microscopic assessment, especially in malignant disease.

The objective of this study is to determine the association of biofilm formation, extended spectrum beta lactamase [ESBL] production and antibiotic susceptibility pattern among uropathogenic Escherichia coli [UPEC] isolates. The present study was conducted on 137 UPEC isolates (counts > 105cfu/ml) from UTI cases. In all these isolates biofilm formation was detected by microtitre plate [MTP] method, ESBL production by combined disc diffusion technique and antibiotic susceptibility testing [AST] by Kirby- Bauer disk diffusion method. The data was analysed by using Medcalc software package. Chi-square test was applied. Among 137 UPEC isolates, 48 (35%) were biofilm producers [BFP’s]and 89 (65%) were biofilm non producers [BFNP’s] by MTP method. Of the total number of137 isolates, the highest number of strains were susceptible to amikacin followed by gentamicin, nitrofurantoin, cefepime.Among 137 isolates, ESBL producers were 28 (75%) of which 21 (75%) were BFP’s also, which makes a total of 49 (35%) (ESBL+Biofilm) and BFNP’s were 7[25%]. ESBL non-producing E.coliwere109 (80%), of which 32 (29.3%) were BFP’s and 77 (70.6%) were BFNP’s. The ability of biofilm formation was significantly higher in ESBL producing strains than that of ESBL non-producing strains (p<0.05). The ability of biofilm formation was found to be higher among ESBL producing stains of E. coli. Higher resistance rate was noted among biofilm producers to almost all the antimicrobial agents except few than non-biofilm producers.

A key characteristic of the adaptive response of cells is that if the stimulus is removed any alteration in cell growth reverts to normal. In contrast to these reversible adaptive responses certain stimuli cause changes in genetic material that result in permanent alteration of the normal cellular growth pattern. Such altered cells, which are termed neoplastic, fail to respond normally to signals controlling the cell growth. They proliferate excessively in a poorly regulated manner, forming a lump or tissue mass called a neoplasm. A tumour is said to be benign when its microscopic and gross characteristics are considered relatively innocent, implying that it will remain localised, cannot spread to other sides. Malignant, as applied to a neoplasm, implies that the lesion can invade and destroy the adjacent structures and spread to distant sites (metastasize) to cause death. Mechanisms by which metastatic cells arise from primary tumours and why they metastasize to specific organs can be explained by cross talk between selected cancer cells (the ‗seeds‘) and specific organ micro environments (the ‗soil‘) implying that Stephen Paget‘s 1889 seed-soil hypothesis. Metastasis of a tumour cell depends on its interactions with the homeostatic factors that promote tumour cell growth, survival, angiogenesis, invasion and metastasis.