Introduction: The Neonatal sepsis defines as blood infection that will occurs in newborn at the first four weeks from birth. Gold standard method is for diagnose of neonatal sepsis that is blood culture but that is time consuming, requires well equipped laboratory and trained laboratory personnel. The study is planned for evaluation of CRP role in the blood as most early marker in neonatal sepsis & find the correlation with blood culture. Materials And Method: The study was done in microbiology laboratory at Sir-T Hospital, Bhavnagar, Gujarat from may, 2020 to October , 2020 to understand the Correlation of C-Reactive Protein and Blood Culture in Neonatal Sepsis. After filling the form of patient’s details in pre-designed proforma the 1-2 ml of blood collected aseptically and its was inoculated into blood culture bottle containing 5 ml of Brain Heart Infusion Broth. CRP level was done by Latex Agglutination Card test. Results: Out of total 481 samples 129 were males and 352 were females. Out of the total 481 samples, 58 were blood culture positive from which 58 were positive for CRP also. Among the blood culture negative samples, 55 were CRP positive. Conclusion: There is Early diagnosis of neonatal sepsis within the aid of biomarkers like C-Reactive Protein may be serve as an important tool in the reducing mortality & morbidity among the neonates.

Les infections respiratoires, virales ou bactériennes, représentent une des causes majeures de surconsommation d’antibiotiques. Les techniques de biologie moléculaire et notamment la PCR Multiplex en temps réel permettent une identification rapide et simultanée d’un large éventail de virus, sous types viraux ainsi que de certaines espèces bactériennes. Cette étude vise à étudier l’épidémiologie des agents pathogènes respiratoires en soulignant l’intérêt de la PCR multiplex dans le diagnostic rapide des infections respiratoires virales et bactériennes. Il s’agit d’une étude rétrospective portant sur 27 prélèvements nasopharyngés reçus au laboratoire de bactériologie-virologie du CHU Hassan II de Fès durant une période de 8 mois allant de décembre 2018 jusqu’à juillet 2019. Les prélèvements ont été traités par la technique PCR multiplex type FilmArray panel respiratoire. Le sexe ratio H/F était de 1,7. La moyenne d’âge était de 15,4. Les services demandeurs étaient respectivement : pédiatrie (63%), réanimation (22%) et urgences adultes (15%).Le panel a détecté 16 cas positif, soit un taux de positivité global de 59%. Les virus isolés étaient comme suit : le humain rhinovirus /entérovirus(8), parainfluenza B (7), influenza A (4), adénovirus (3) et enfin coronavirus(1) et virus respiratoire synticial(1). L’utilisation de PCR multiplex type FilmArray panel respiratoire permet d’améliorer le diagnostic étiologique .elle permet aussi une meilleure maitrise du risque infectieux en identifiant les patients sujets à un isolement et en démarrant précocement un traitement antiviral adéquat réduisant de la sorte les prescriptions inappropriées d’antibiothérapie. La rapidité de rendu des résultats offre au clinicien une décision d’hospitalisation mieux documentée et au patient une durée de séjour plus faible. En dépit des vertus de cette technique, un problème d’accessibilité rencontre les cliniciens vu son cout élevé.

Wilms tumours with a greater number of heterologous elements on histology are considered as teratoid form of Wilms tumour. The presence of teratoid components in Wilms tumour are resistant to chemotherapy and may warrant additional treatment. On microscopy, the epithelial component in Wilms tumour most commonly present in form of tubules or glomeruloid bodies as homologous differentiation and less commonly as heterologous elements such as squamous or mucinous epithelium. Other common heterologous elements described in the literature are skeletal muscle, smooth muscle, adipose tissue and cartilage. Though the transitional lining is homologous to the kidney, nests of urothelial cells within Wilms tumour is not described well in the literature. We describe the presence of urothelial nests intimately admixed with other homologous and heterologous components of Wilms tumour in post-chemotherapy nephrectomy specimen.

In the pathogenesis of rheumatoid arthritis and primary biliary cirrhosis, the tumor necrosis factor-alpha has an important role. We describe the case of a 61-years-old woman with rheumatoid arthritis and primary biliary cirrhosis treated with anti-tumor necrosis factor-alpha agents. During treatment with golimumab, we found an improvement in liver function, but rheumatoid arthritis remained poorly controlled. When etanercept was started, the disease activity of rheumatoid arthritis was significantly improved and liver function was also improved despite the appearance of Pityriasis Rosea during etanercept. This case shows that etanercept therapy maintained liver enzymes within normal limits in PBC and controlled arthritis with a 10-month follow-up but the maintenance of this treatment was limited by the appearance of the pityriasis rosea.

Background: Helicobacter pylori infection of the gastric mucosa is the most important aetiologic agent identified to cause chronic gastritis with consequences ranging from acute ulceration to the development of debilitating gastric cancer. Histopathologic characterisation of the disease progression from gastric biopsies is frequently required. Objectives: To determine the histopathologic pattern of gastric biopsies categorised chronic gastritis using updated Sydney classification and determined the role of Helicobacter pylori in chronic gastritis in our centre. Methodology: A retrospective study of all the gastric biopsies received in the department of Histopathology, Usmanu Danfodio University Teaching Hospital, and Sokoto between the years 2011-2020. Formalin fixed, paraffin embedded tissue blocks and slides stained with routine, special and immunohistochemical stains were used. Microscopic features detailing different pathologies were analysed and chronic gastritis were classified based on updated Sydney classification. Information on age, sex and duration of disease was retrieved from request cards and case folders. Collected data analyzed using SPSS version 20. Results: There were 252 gastric biopsies received, 137 male and 115 female patients and a ratio of 1.2:1. Their ages ranged from 16 to 82 years and peak in the 6th decade of life with a mean age of 46.5 years. The most common diagnosis was chronic gastritis and constitutes 85.5% (215 cases), followed by adenocarcinoma (10.3%), gastrointestinal stromal tumour (2.4%) and gastric ulcer (2.0%). Among chronic gastritis, 47.4% were H. pylori associated. Moderate to severe activity and inflammation were seen in 45.6% and 48.4% respectively. Mucosal atrophy (Atrophic Gastritis) was seen 81.4% while 19.5% show complete or incomplete intestinal metaplasia. Conclusion: Helicobacter pylori organism was the common cause of gastric morbidity in our environment, causing chronic gastritis with predominantly mild to moderate grade of inflammation, mucosal atrophy, activity and organisms colonisation.

Background: Peripheral blood plasmacytosis can be seen in plasma cell leukemia (PCL) or plasma cell myeloma (PCM). As plasma cells show dysplasia or lymphocytoid morphology, they masquerade as monocytes or high fluorescent lymphocytes (HFL) on automated cell analyzer. The early suspicion and detection are of clinical importance for diagnostic and prognostic reasons. Methods: Automated cell analyzer was used for routine CBC examination. Peripheral blood smear examination was performed for enumeration and characterization of cells in peripheral blood followed by bone marrow examination and ancillary techniques. Conclusion: Lymphocyte-Monocyte peak with increased high HFL count on CBC should prompt a smear examination of the peripheral blood for circulating plasma cells and immunohistochemistry markers like CD38, CD138, CD56 should be used to establish the clonality in cells.

The skin, genital system, and hematological involvement of diffuse large B-cell lymphomas are quite rare. Diagnosis and disease classification are based on histological review and immunohistochemical staining. PCDLBCL is an aggressive lymphoma with an inferior prognosis. However, timely recognition can have important clinical and therapeutic implications.

Mycetoma is a chronic, suppurative, granulomatous infection of subcutaneous tissue that eventually may involve bone. The Infection is caused by free living, exogenous, geophilic actinomycetes and fungi. It is characterized by a triad of localized swelling, underlying sinus tract and production of grains or granules. Approximately 40% of mycetomas worldwide are eumycotic and rest is actinomycotic [1]. Eumycetoma is most probably acquired by traumatic inoculation of certain fungi into subcutaneous tissue. Fungi are considered as an unusual cause of osteomyelitis of hand/palm. This paper describes a case of eumycetoma osteomyelitis of the hand. With clinical, radiological and pathological correlation we arrived at a final diagnosis.