Papillary Lesions of Breast, defined histologically by presence of fibrovascular cores with varying epithelial proliferation, encompass a wide spectrum of benign intraductal papilloma, atypical papilloma with ADH/DCIS, papillary DCIS, encapsulated papillary carcinoma, solid papillary carcinoma and invasive papillary carcinoma. Due to tumor heterogeneity, sub-classification is diagnostically challenging on histopathology alone thus requiring help of immunohistochemistry (IHC). The aims & objectives of this study was to assess papillary lesions of breast and to determine the histopathological features which can categorize various papillary lesions along with IHC.A retrospective analysis of 39 cases of papillary breast lesions retrieved over a period of 8 years (July 2011 to July 2019) was done. The histopathology was reviewed independently by two pathologists using a standard review form which included 10 parameters. IHC was applied on all the cases to confirm or refute the histopathological diagnosis. Statistical analysis were performed using PRIMER software. Out of 39 cases, the most common papillary breast lesion was benign intraductal papilloma with 28 cases (72%), followed by one case of atypical papilloma with DCIS (2%) and 10(26%) malignant papillary lesions. The statistically significant histopathological features which aided in differentiating benign lesions from malignant lesions were presence of apocrine metaplasia, bland nuclear features and absence of atypia. IHC was necessary in diagnosing all the malignant papillary lesions and 2 benign papillary lesions. Papillary lesions of breast are difficult to interpret on microscopy alone due to intrinsic heterogeneity. The combination of histopathological features along with IHC helps in distinguishing benign, atypical and malignant papillary lesions.

Newcastle disease is caused by paramyxo viruses, very infectious disease present in birds harms most. Clinical and electro-physiological findings in the patients of SMA revealed that the patients experience critical decline in the functions of motors with unit loss rapidly. Tumors of heart in dogs typically represents either non-functional chemodectoma or ectopic thyroid carcinomas which is also non-functional. Spontaneous-malignant-lymphoma present in sheep, through well known in tumor investigations in the slaughtering houses and in the diagnostic workshops. The hallmark clinical finding in cases of external caseous lymphadenitis is the development of abscesses in the region of peripheral lymph nodes. Anthrax is a disease which is usually spread by feeding and by the contaminated water with the spores and these spores can last in the soil for several years. Mostly the anthrax is cured easily in start stages and is easy way and control to further increase of this disease. Orf is the disease commonly present in sheep and goats throughout the world. It also known as scabby mouth or sore mouth. The outlook for Orf sickness, which is a zoonotic host switch infection, is not especially bad. There are different parasitic diseases of animals, coccidiosis is one of the parasitic disease of poultry and rabbits.

Plant responses to various stresses are complex, involving alterations in the physiological, cellular, and transcriptome levels. Polyamine accumulation in diseased tissues is difficult to identify as it is found in both pathogenic fungus and plants. Abiotic stressors, as a group, pose a serious challenge to the ecosystem and agriculture also responsible for significant agricultural production losses. Benzoxazinoids are plant secondary metabolites found in grasses that have a significant potential for chemical defense against biotic stresses seen across the animal world. EREBP (Ethylene Response Element Binding Protein) transcription factor gets highly expressed in association with the ROS scavenging system, with decreased expression of the dehydrogenases gene. Crop productivity is also affected by biotic stresses. Plants physiological characteristics are altered by drought, excessive heat, and with their combined effect. Heat and drought stress have been proven to affect photosynthetic activity. Hydroponic systems operate by allowing precise control of environmental factors such as pH-balance and temperature, as well as increased nutrient and water exposure.

Background: Skin lesions are commonly encountered worldwide, but they differ in their clinical presentation depending on demographic region. It is essential to diagnose them correctly as management differs for each. Thus, histopathology is required as a definitive diagnostic tool. Aims and Objectives: To study histopathologic spectrum of non-neoplastic skin lesions, to determine age and sex distribution and analyse the histopathologic subtypes of these lesions. Material and methods: 102 skin biopsies received in the histopathology section of the Department of Pathology of a tertiary care hospital in Northern India were studied over a period of 2 years. 5micron thick sections were done and routine staining with haematoxylin and eosin was done in all the cases. Special stain was applied as and when required. All data was recorded carefully and represented in the form of tables/ charts. Results: A total of 102 skin lesions were analyzed, of which 47.1% were detected in age-group 21-40 years and 64.1% being males. Clinically, hyperpigmented patches/plaques were most frequently encountered. Psoriasis was most common histopathologic diagnosis (39%) followed by Hansen’s disease (17%), with tuberculoid leprosy being the most common subtype. Conclusions: Histopathological examination of skin biopsy remains the gold standard technique for diagnosing a variety of skin lesions. In correlation with clinical history it aids in the accurate diagnosis of the majority of the skin lesions.

Introduction: Infection with the metallo-beta-lactamase (MBL) producing organisms is associated with higher rates of mortality, morbidity, and health care costs. MBL producing Pseudomonas aeruginosa was first reported in Japan in 1991 and since then has been found in various parts of the world. Objective: To Assess the Dissemination of Class 1 integron among different MBL producing Acinetobacter baumannii In ICU of DMCH, Dhaka, Bangladesh. Methodology: This Cross sectional study was conducted between July 2013 to June 2014 in the department of Microbiology, Dhaka Medical College (DMC), Dhaka, Bangladesh to determine the frequency and susceptibility patterns of MBL-producers among carbapenem-resistant Gram-negative rods (GNRs) from clinical isolates of a tertiary care hospital. All clinical samples were processed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to the detection of MBL production by the E-test MBL strip method. Hospitalized patients who did not give consent were excluded in this study. Data regarding age, sex, duration of hospitalization, antibiotic history were collected using a prescribed data collection form. Results: Out of 22 imipenem resistant Acinetobacter baumannii. 21 (95.45%) were positive for MBL production. The distribution of MBL genes among imipenem resistant Acinetobacter baumannii. Out of 22 imipenem resistant strains, 21 (95.45%) were positive for blaNDM-1, 20 (90.91%) for blaNDM-like, 16 (72.72%) for blaVIM and all the 22 (100%) were negative for blaIMP and distribution of class I integron among the MBL producers. Among the 21 MBL producing Acinetobacter baumannii, 17 (80.95%) had class I integron. This study presence of class I integron and conserved segment of class 1 integron among the different MBL producing Acinetobacter baumannii. Among 16 blaVIM positive Acinetobacter baumannii. 13 (81.25%) carried class 1 integron and 11 (68.75%) carried conserved segment of class I integron. Out of 21 blaNDM-1 positive strains. 17 (80.95%) carried class I integron and 13 (61.9%) carried conserved segment of class 1 integron. Out of 20 blaNDM-like positive strains, 16 (80%) carried class 1 integron and 12 (60%) carried conserved segment of class I integron. Conclusion: The findings strongly suggest that there is a need to track the detection of MBL producers and that judicious use of imipenem is necessary to prevent the further spread of these organisms.

Background: Urinary tract infection (UTI) is among the most common bacterial infections and possess significant healthcare burden. Escherichia coli is the most common cause of UTI accounting for about 70%% and a variable contribution from Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella pneumoniae Patients are often treated as soon as bacteria are shown to be present by microbiological culture. Objective: To assess THE PCR results among culture positive and culture negative specimens of suspected UTI patients. Methods: This study was carried out in the department of Microbiology, Mymensingh Medical College during the period from July 2016 to June 2017. Urine specimens were collected and isolationand identification of major uropathogens (Escherichia coli Klehsiella pneumonias, Proteusmirabilis, and Pseudomonas aeruginosa) were done by standard microbiological procedure a biochemical tests. PCR was performed by using standard protocol with species specific primer for detection of fimH gene for Escherichia coli, fimK gene for Klehsiella paeunomiae, UreCfor Proteus mirabilis, ETA for Pseudomonas aeruginasa. Results: Out of 250 urine specimens, 200 specimens were isolated and identified by culture and different biochemical methods which were supported by microscopical examination and at the same timePCR could detect species specific genes in 201 specimens directly from urine of suspected UTIpatient Escherichia coli was responsible as a leading causative pathogen in both outpatient department and in patient department with a higher prevalence of 71.8% for outpatient department. On the other hand Pseudomonas aeruginosa Profeus mirabilis and Klebsiella pneumonia were more prevalent in in-patient department and it was 21.1%, 5.6% and 5.5% respectively, Among the 50 culture negative urine specimens, 14 (28%) showed PCR positive for Escherichia coli, Klehsiella pneumonia and Pseudomonas aeruginosa. Conclusion: This study revealed that, the prevalence of UTI is high n MMCH Single pathogen base uniplex PCR was found superior than standard culture and less time consuming. Because uniplex PCR could detect many (28%) culture negative cases.

mucormycosis associated with COVID-19 is now become a serious health concern around the globe, including several Asian countries. In Pakistan mucormycosis fatalities are now being found among COVID-19 individuals. Individuals with diabetes, malnourishment, Cancer, organ transplantation, active tuberculosis, Liver diseases, chronic respiratory diseases, HIV, AIDS and asthma are more Susceptible to infection. Diabetes mellitus patients are at more risk of mortality infection of this fungus. To counteract mucromycosis in patients, rapid and precise diagnostic facilities, medical assistance, and a quick yet coordinated approach are all suggested.

Aims: Thyroid gland is composed of spherical follicles and thyroid parenchyma includes two major cell types, the thyrocytes releasing thyroid hormones and C cells secreting mature calcitonin. Hypothyroidism and Hyperthyroidism both have been, known to be associated with the various abnormalities of the coagulation system. Our study, had been purposed to investigate the relationship between Mean platelet volume, platelet distribution width and plateletcrit with T3, T4, TSH levels of the patients. Methods: A retrospective evaluation was conducted on 90 patients (age group from 30 to 45 years) diagnosed with hypothyroidism (n=30), hyperthyroidism (n=30) and euthyroid (n=30) for over 6 months from October 2020 – March 2021. The demographic data and T3, T4, TSH values was obtained from the patients lab reports in Saveetha Medical College and Hospital. These values was correlated with platelet indices of MPV, PDW and PCT obtained by Sysmex-XN 1000 automated analyser. Result: Females population was predominant in both hypothyroidism and hyperthyroidism. On comparison of hypothyroid patients with euthyroid revealed statistically increase significant difference in plateletcrit. On comparison of hyperthyroid patients with euthyroid revealed statistically significant increase in Platelet count, MPV and plateletcrit. Conclusion: The present study, suggest that platelet parameters can be considered as a reliable markers and can be used as CVD risk evaluation parameters in hypothyroid and hyperthyroid patients due to abnormal thrombovascular activity.

Introduction: In the modern world, there is a rising trend of diabetes mellitus and hypertension in developing countries like India, which is favouring a rise in complications like Chronic kidney disease.Thrombotic complications are a high possibility in chronic kidney disease (CKD). In recent days, abnormalities in platelet parameters are found to be an effective tool in risk stratification of CKD patients to develop venous thromboembolism and vascular disease. Our study was conducted to assess relation of platelets indices, platelet count, mean platelet volume (MPV), platelet distribution width (PDW) and plateletcrit (PCT) in CKD patients. Material and methods: A retrospective evaluation was conducted on the blood biochemical data and hematological data obtained from the records of patients diagnosed with chronic kidney disease for over 5 months (January 2021- May 2021) . The demographic data, urea, creatinine values and hematological findings were collected from the patient lab reports from the Department of Biochemistry and Pathology of Saveetha medical college and hospital. Platelet indices were obtained using Sysmex -XN 1000 automated analyser. 131 cases of known CKD was collected and correlated with Platelet indices values. Results: On gender and age distribution, male patients were predominant and age group between 41 to 60 were commonly affected. In CKD stage 2-39 patients, stage 3-32 patients, stage 4-28 patients, stage 5-14 patients were categorized according to their clinical findings and laboratory investigations. On correlation, there were no statistically significant differences in any PLT indices (platelet count, mean platelet volume, platelet distribution width and plateletcrit).However, Platelet count lower as the stage increases and MPV, PDW and plateletcrit were slightly higher in stage 5 CKD when compared with other stages of CKD. Conclusion: Platelet indices plays major mechanism in pathological processes of vascular thrombosis. The efficacies of platelet indices associated with CKD patients remain unknown. Prospective randomized controlled trials involving larger numbers of CKD patients are needed to determine the associations with platelet indices.

Background: Chronic renal failure (CRF) patients with superseded diabetes are immune-compromised and are prone for oral candidal infection. Aim: To study the Antifungal drug susceptibility of oral candida species isolates in chronic renal failure patients with type 2 diabetes. Material And Methods: A total of 98 individuals including 73 cases of chronic renal failure with type 2 diabetes mellitus and 25 healthy individuals as controls. The diabetic patients were divided into 3 groups according to their glycemic index; 22 controlled diabetes (HbA1c ≤5%), 27 moderately controlled (HbA1c 5-7%) and 24 Uncontrolled diabetes (HbA1c ≥7%). Salivary samples were collected in as sterile container with phosphate buffered saline and then immediately transported to various mycological investigations and antifungal susceptibility tests. Results: There was significant difference in incidence of candida species in uncontrolled diabetes when compared to moderately controlled, controlled and normal patients (P<0.05).The higher number of colony count was seen among uncontrolled and moderately controlled diabetes than controlled and healthy subjects. Candida albicans is the most prominent species among the groups. C. albicans showed increased resistance to Amphotericin B and fluconazole in diabetic patients in comparison to control group. Other species showed variable sensitivity patterns. Conclusion: An accurate identification of oral candida species and their drug susceptibility, in chronic renal failure with type 2 diabetes patients mandates proper treatment to avoid recurrence and drug resistance.

Introduction: Wound infection remains an important cause of morbidity and mortality among humans, especially in developing countries. Knowledge regarding Bacterial isolates and their antibiotic susceptibility pattern in pus isolate is necessary for empirical treatment of wound infections and useful for making antibiotic policy of hospital. Aim: The present study was conducted to assess bacterial isolates and their antimicrobial susceptibility patterns from pus samples of Sir T Hospital, Bhavnagar. Material and method: The present study was carried out during July 2019 to November 2019 in Microbiology Department, Sir T Hospital, and Bhavnagar. The pus samples received in bacteriology section of microbiology laboratory were preceded for bacterial identification and antibiotic susceptibility testing. It was done by Modified kirbybauer disk diffusion method according to CLSI guidelines 2019. Result: A total of 1110 pus samples were received from which 477 (42.97%) samples were positive for gram positive 117(24.52%) and gram negative 360 (75.47%) bacteria. About 117(24.52%) of the total isolates were Staphylococcus aureus, 220 Escherichia coli (41%), 96 Klebsiellaspp (17%), 16 Pseudomonas (13%), 13 Proteus mirabilis (2%), 11 Proteus vulgaris (2%), 4 Acinatobacter species (0.74%). Gram negative organisms were sensitive for Meropenem (98%), Piperacillin Tazobactem (77%) Gram positive organism were completely sensitive to Vancomycin (100%), Linezolid (100%). Conclusion: Thus the present study shows that Escherichia coli, Klebsiellaspp, Pseudomonas and Staphylococcus aureus are the most common bacteria showing sensitivity towards vancomycin, linezolid, meropenem, piperacillin tazobactum. This study helps in decidiing proper treatment of wound infection.

Introduction: Streptococcus pyogens is the commonest human pathogen causing widespread infections. Streptococcal antibody tests like the antistreptolysin O titre (ASO), the antideoxyribonuclease-B titre (anti-DNAase-B, or ADB) and the streptozyme test can be used for diagnosis of streptococcal infections. ASO test is done using latex agglutination method. Aim: To detect prevalence of anti-streptolysin O (ASO) antibodies in serum samples received in microbiology laboratory from patients of suspected streptococcal infections. Material and Methods: This study was conducted in serology section of Microbiology laboratory, Sir Takhtsinhji Hospital, Bhavnagar between the periods of June 2019 to October 2020. Serum samples received in serology section of microbiology requested for Anti streptolysin O test were tested by ASO- latex slide agglutination test by Pathozyme Diagnostics. Total 476 serum samples were tested. The results were recorded and analysed for the study. Results: Total prevalence for ASO positive serum samples were 46(9.8%), the highest prevalence 15% found in age group of 0-20 years. Conclusion: For detecting antecedent streptococcal infection the estimation of ASO antibodies is a simple, cost effective way. The increased level of ASO can support the diagnosis of rheumatic fever. ASO prevalence was found to be highest in the 0-20 year’s age group.

Cancer biomarkers are the biological molecules produced by the body or tumor in a person with cancer. In order to place the functioning of biomarkers for clinical investigating, they are passed through different clinical trials in order to check their treatment rate as compared to the normal markers. Different genes are involved in causing the breast cancer and early diagnosis through biomarkers provides an effective way to control the mutations caused in cancerous tissues. Genetic biomarkers are those biological molecules that can detect the change in the DNA and RNA structures. HER2 somatic mutations lead to increase in progressions of cancer development non-small cell lung cancers as well as in breast cells. The most important biomarkers are ALF-alpha-fetoprotein is a protein that in humans is encoded by the AFP gene in the liver. Mutational defect in AFP gene leads to severe damage to liver. C-reactive protein (CRP) is the biomarker for inflammation in the body cells. Prostate specific antigen (PSA) is the biomarker used for detection of prostate cancer. Microsatellite instability analysis of a tumor which provides predictive and also prognostic information. Cerebrospinal fluid (CSF) is the important biomarker for the diagnosis of dementia, Alzheimer’s disease pathologies.

Aim: To study the demography, maternal and fetal causes of chorangiosis. Study design: A retrospective descriptive study. Place and Duration of Study: Total of 73 patients with chorangiosis were studied in Department of Pathology Saveetha Medical College, during the period of August 2020 and February 2021. Methodology: Study included 73 patients of placental chorangiosis. The clinical history and demographic data was obtained from the patient’s medical records. Thematernal and fetal causes were studied, and clinico-histopathological correlation was done. Results: This study includes 73 postnatal women, of whichthe rate of cesarean section was noted to be 69.8% and the mean value were obtained showed mean gestational age of 37.5 weeks, 70 live births and 3 neonatal deaths; mean placental weight is 480 grams and birth weight 3970grams. The mean 1 minute and 5 minutes APGAR score is reduced indicating the disturbance in fetal vitals immediately after birth. Conclusion: Chorangiosis is a rare illness, which is associated with an increased risk of prenatal and neonatal morbidity and mortality. So, it should be regarded as a key prognostic indicator of poor pregnancy outcomes and should be essentially reported in the histopathological evaluation.