Altered Total Antioxidant Capacity and Malondialdehyde in Cervical Cancer Patients and Effect of Chemoradiation
Shah SR, Shaheen B Shaikh, Shaheena Yassir
Page Numbers : 14-19
DOI : 10.36348/sijb.2021.v04i2.001
Background: One of the leading causes of cancer-related death in women worldwide, that causes an enhancednegative impact on quality of life with regard to social and economic burden, is Cervical cancer (CaCx). Early diagnosis and treatment can plummetassociated mortality and morbidity, by hit hard and early approach. Aim and objective: To estimate serum total antioxidant capacity (TAC), and malondialdehyde (MDA) in CaCx patients and analyse their response to chemoradiation. Materials and Methods: Histopathologically proven CaCx patients (n=50) and age-matched healthy females (n=50) were recruited in case-control study. Estimation of Serum TAC, and MDA was done in recruited subjects. Both of these parameters wasestimated again after chemoradiationin CaCx patients, to scrutinize the effect of chemoradiation. In addition, 84% controls and 34% cervical cancer patients had a history of Vit. C and E supplementation. Results: The mean +SD age of the patients and controls was 43.98±6.38 and 31.56±6.84 years, respectively. The mean level of serum MDA in the patients was significantly higher as compared with the controls, whereas the mean TAC in the patients was reduced in same comparison. After chemoradiation, serum levels of TAC and MDA, increased and decreased, respectively. Conclusion: These analytical data captured suggests that patients with CaCx were in oxidative stress because the chosen oxidative parameters, serum MDA were increased, and the defensive TAC was decreased in them. Chemoradiotherapy improved their antioxidant capacity. Further studies are needed to evaluate the concurrent use of antioxidants with chemoradiotherapy for improving the disease prognosis.
Original Research Article
Feb. 27, 2021
Cymbopogon citratus 80% Methanolic Leaf Extract Inhibit Acetyl-Cholinesterase on Mice Model
Ibrahim Maina Hassan, Bulama Ibrahim
Page Numbers : 6-13
DOI : 10.36348/sijb.2021.v04i01.002
Oxygen is a unique element indispensable for life. Free radicals are forms as a consequence of ATP (adenosine triphosphate) production by the mitochondria in oxidative phosphorylation due to use of oxygen by cells to generate energy. Most of the by-products form as a result of this biochemical changes are reactive oxygen species (ROS) as well as reactive nitrogen species (RNS). Delicate balance between the two antagonistic effects is among the vital aspect of life ROS and RNS exert beneficial effects on cellular responses and immune function at low or moderate concentration. Therefore, the aim of this study is to evaluate the cholinesterase inhibitory effect of Cymbopogon citratus 80% (leaf) extract. The leaf was extracted with 80% methanol. A toxicity study was carried out on mice. Cholinesterase inhibitory activities were also evaluated on the same mice using Ellman’s method. Result revealed high cholinesterase inhibitory activities of the crude extract with high significant differences at P<0.001) between the group that were treated with crude extract only, group treated with crude extract and exposed to arsenic and group that were exposed to arsenic only as well as group that are maintained in complete media. It can be concluded that low toxicity and high cholinesterase inhibitory effect of the crude extract is responsible for it therapeutic effects of this crude extract. Toxicity screening of this crude extract on a mammal such as rat to reaffirm their toxicity profile are recommended. Antioxidant screening as well as isolation of bioactive compounds present in this plant part is strongly recommended.
Feb. 23, 2021
Phyto-Pharmacological Potential of Zizyphus jujube: A Review
Himesh Soni, Jitender K Malik
Page Numbers : 1-5
DOI : 10.36348/sijb.2021.v04i01.001
Natural world has been a resource of curative agents for thousands of year and an inspiring number of modern drug have been isolated from natural sources, many based on their use in traditional medicine. Plants from the genus Zizyphus have been used in conventional medicine by many cultures. Flavonoids, phenolic compounds tri-terpenic acids and polysaccharides constituents have been reported as the major phyto-constituents of the Zizyphus species. This review describes pharmacological reports of the Z. jujube. Various virgin areas of investigate on the Z. jujube have been spotlighted with a sight to explore, isolate and identify the medicinally important phyto-constituents which could be utilized to alleviate diverse diseases distressing the mankind.
Original Research Article
Dec. 30, 2020
Epidemiological Risk Factors Influencing the Formation of Renal Calculi, their Chemical Composition and Association with Urinary Tract Infections
P. Sri Lakshmi, Kalyan Kumar Kakarla, Pendru Raghunath, Y. V. Ramakrishna Reddy
Page Numbers : 260-266
DOI : 10.36348/sijb.2020.v03i12.005
This study was conducted to assess the influence of epidemiological risk factors in the formation of renal calculi, their compositionand association with urinary tract infections (UTI) among the ethnic population of Khammam district, Telangana state. This study included 56 subjects attending the urology department, Mamata General Hospital, Khammam. Out of 56 patients with renal calculi, 50 are male, 46 from rural areas, 22 were farmers and 26 were manual labor. Twenty six belonged to socioeconomically lower middle class, 38 were non-vegetarians and 47 were consuming bore/tank water. Results of this study suggest a significant association between all the epidemiological risk factors viz., gender, residence, occupation, socio economic status, diet, and source of water and formation of renal calculi. Structural analysis of stones demonstrated that 78.6% of stones were mixed. Overall, calcium oxalate as pureor mixed with otherchemicals was the main component of stones matrix, seen in 38 (67.9%) patients. In this study, struvite is present in 21.4% of the stones. Thirty (53.6%) of 56 urine specimens were culture positive and 26 (46.4%) were sterile. All the positive urine cultures yielded a single organism and 26of 30 isolates were positive for urease enzyme. Klebsiella was the most common organism and was isolated from 16 (28.6%) urine samples. Results suggest that there is a significant association between UTI and renal calculi formation.
Original Research Article
Dec. 25, 2020
Epidemiological Profile of Hyperferritinemia in the Military Hospital Moulay Ismail of Meknes, Morocco
M. Mahha, R. Hendi, A. Laraqui, N. El Mouh, L. Balouche, Y. Bamou, F. El Boukhrissi
Page Numbers : 255-259
DOI : 10.36348/sijb.2020.v03i12.004
Introduction: Hyperferritinemia occurs in several medical conditions. The most prevalent underlaying causes are kidney and liver diseases, infections and tumors. The objective of our study was to establish the epidemiological profile of hyperferritinemia among patients consulting cases who attends the Military Hospital Moulay Ismail of Meknes. Materials and methods: In this study, we have enrolled all patients whose blood level of ferritin are above 600µg/l measured on Cobas® 6000 Analyzer, during a period of 12 months. Results: A total of 413 patients had values greater than 600 μg/l, which corresponds to a percentage of 7.37%. The average age was 44 years ± 8 years (18-70). The H/F sex ratio was 1.27. The mean rate of ferritin serum level was 1123 µg/l. Patients with hyperferritinemia were mostly outpatients in 76.7% of cases. Referred patients comes from internal medicine department (9.4%), nephrology (7%), gastrology (3.2%), oncology (2.7%), endocrinology and hematology (2.4%), dermatology (1.9%) and rheumatology (1.2%). The distribution of etiologies was as following: infections (56%), nephropathies (31.5%), systemic diseases (17.5%), liver diseases (15%), diabetes (8.5%), hemopathies (7.5%), hemochromatosis (7%) and neoplasia (5%). More than the half of patients (57%) had multiple causes simultaneously. The most common causes associations in patients with hyperferritinemia were respectively: infections-nephropathies (24%), infections-systemic diseases (14%) and infections-nephropathies-diabetes (6.5%). Discussion and Conclusion: Infections and kidney diseases dominated the etiological spectrum of hyperferritinemia in our population. However, the underlying causes remain various, which makes hyperferritinemia a poor specific marker for disease diagnosis.
Original Research Article
Dec. 20, 2020
To Estimate Trace Elements, Oxidative Stress and Antioxidant during Preeclampsia and Eclampsia
Sonali Rajdev, Dr. B.K. Agarwal, Dr. Shreya Nigoskar
Page Numbers : 250-254
DOI : 10.36348/sijb.2020.v03i12.003
Introduction: Pregnancy is characterized by significant metabolic and hemodynamic changes that begin early in the gestational period. Major hemodynamic changes include an increase in the cardiac output during the first trimester, sodium and water retention leading to plasma volume expansion with a peak around week 30, and reductions in the systemic vascular resistance and systemic blood pressure. Material and Methods: This is prospective and observational study conducted at Department of Biochemistry in association of Department of General Medicine. In this study investigation was done to determine the contribution of different biochemical parameters of females suffering from preeclampsia and Eclampsia. Results: Serum iron level in case group 18.43 ± 8.49 μmol/L and control group 15.92 ± 8.23. Moreover, Serum Zinc level 8.4 ± 1.9 μmol/L and control group 9.3 ± 1.2 μmol/L. Furthermore, Serum Copper level 17.3 ± 5.3 μmol/L and control group 9.6 ± 1.9 μmol/L. Oxidative marker between Case and control group was statistically significantly difference. MDA in case group was 3.18 ± 0.43 nM/mL and Control 1.63 ± 0.32 nM/mL. Antioxidant marker between Case and control group was statistically significantly difference. In case group Glutathione peroxidase 9.53 ± 2.46 μmol/ml and control group 11.68 ± 3.63 μmol/ml. Conclusion: The results obtained support our initial hypothesis that common differences between all pregnant women and controls could be revealed, while preeclampsia and the other pregnancies would further differ indicating specific misbalance of the oxidative stress in preeclampsia.
Original Research Article
Dec. 13, 2020
Association of Hemoglobin Levels with Stroke Mortality in Patients of Type 2 Diabetes Mellitus
Dr. Shaheen B Shaikh, Dr. Ismail HM, Miss Roshni, Dr. Shaheena Yassir, Dr. Saiqa R, Dr. Sarfaraz Shaikh
Page Numbers : 246-249
DOI : 10.36348/sijb.2020.v03i12.002
Background: In stroke scenario, decreased hemoglobin levels have the potential to worsen brain ischemia; however, the association between the levels of hemoglobin with stroke mortality is not well understood. Aim: The study investigated the association of hemoglobin levels with 28 days mortality rate after ischemic stroke (IS). Methods: This was a retrospective study, where data of 74 patients admitted to ICU was collected with a diagnosis of stroke .The data were analyzed in the terms of demographic details, plasma glucose, HbA1c and hemoglobin levels. Results: Among 74 analyzed patients, 56.70% were men and 43.30% were females. In our study 32.4 % of stroke patients had anemia. Out of 45.9% non survivors, 21.6% stroke patients had hemoglobin level less than 11mg/ dl and 24.30% stroke patients had hemoglobin level more than 11mg/dl. Out of 54.1% survivors ,10.8.% and 43.20% stroke patients had hemoglobin level less than 11mg/ dl and more than 11mg/dl, respectively .The data was statistically significant. Conclusion: In conclusion, we showed that a significant proportion of stroke patients have low hemoglobin at the time of stroke onset and this is associated with increased mortality up to 28 days. Targeted interventions in this patient population may improve outcomes and require further evaluation.