ORIGINAL RESEARCH ARTICLE | Feb. 15, 2018
Extracellular Enzymatic Assays of Lipolytic Bacteria on Lipid-Rich Abattoir Waste water
Ononiwu Prosper Ikechukwu, Ekwenye, Uchechi N
Page no 99-104 |
10.21276/haya.2018.3.2.1
Wastewater from abattoirs has great impact on the environments, since it is
made up of a significant portion of organic contents such as lipids containing high
BOD and COD. Amongst other sources of lipid-rich wastewater, in the abattoir
wastewater, a significant amount of bacterial capable of utilizing lipids and other
organic contents has been found. Exploiting the extracellular hydrolytic capabilities
of these microbes is necessary for the overall reduction in the organic contents of the
wastewater. Lipolytic bacterial cultures of Pseudomonas aeruginosa, Staphylococcus
aureus, Klebsiella pneumoniae, and Bacillus subtilis were used in the treatment of the
lipid-rich wastewater with enrichment nutrient combinations. Thus, the extracellular
enzymes; lipase, protease and amylase were assayed at 6hour intervals for a period of
48hours for hydrolytic activity. Bacillus subtilis treatment showed the highest
amylase and protease activity while Pseudomonas aeruginosa showed the highest
lipase activity and high amylase and protease activities with Staphylococcus aureus,
also a good lipase producer. The result indicated extracellular production of the
enzymes to cater for the initial breakdown of substrates in the wastewater medium.
Owing to the enzymatic activities of lipase, protease and amylase possessed by these
lipolytic organisms, they could prove to be beneficial for bringing down the overall
organic load of abattoir wastewater.
ORIGINAL RESEARCH ARTICLE | Feb. 15, 2018
Study of Haemoglobin Level and Tumour Growth on Mouse Ascites Tumour in Response to Combination Effect of 2-Methoxyestradiol and Cyclophosphamide
Srabantika Mallick, Samarendra Nath Banerjee, Goutam Paul
Page no 105-110 |
10.21276/haya.2018.3.2.2
S-180 tumour bearing mice were subjected to 2-Methoxyestradiol (2ME)
and Cyclophosphamide (CP) monotherapy and 2ME and CP combination therapy on
7th day of ascetic tumour cell transplantation when the tumour growth was at log
phase. Then, the effect has been studied on host’s system in respect to dead cell –
living cell frequency, tumour volume, haemoglobin percentage, and differential count
of WBC. In 2ME and CP combination therapy, a steady increase in the dead cell or
non-living cell population was noted with the steady decrease in tumour volume.
Haematological studies from peripheral blood revealed a drastic depletion in
neutrophil count and elevation of lymphocyte population on the 12th day and 16th day
of tumour transplantation in combination therapy series. Moreover, the haemoglobin
concentration is more or less stable in combination therapy treatment series. So, the
2ME and CP combination therapy provides some protective compensatory
mechanisms in the body of the host.
ORIGINAL RESEARCH ARTICLE | Feb. 15, 2018
Bark Extravitism and Health Maintenance among Ijaw Tribal Community of Bayelsa State, Nigeria
Eunice Damiloa Adedeji, Joshua Kayode, Ayodele Oyedeji, Mary Kehinde Olanipekun
Page no 111-120 |
10.21276/haya.2018.3.2.3
The study examined plant species whose barks were being extracted and
used for health maintenance by the Ijaw tribal community of Bayelsa State of
Nigeria. 150 respondents, randomly selected, were interviewed with the aid of a
semi-structured questionnaire guide. All the interviewed were focused, conversational
and two-way in communication. Plant species with medicinally valued barks were
identified and information on them were obtained and documented. The results
obtained revealed that the socio-economic status of the respondents were not prerequisites to their consciousness on the medicinal values of plants in their vicinities.
Despite the availability of most of the species in the respondents’ environment,
market patronage was still the preferred source as many of them could not recognize
the species even with their vernacular names. Also a declining rate in the passage of
ethno-botanical knowledge from one generation to another now abounds in the study
area. Trading in medicinal barks was prominent and was dominated by the females
who sourced their stocks from diverse areas. A total of 62 plant species, belonging to
38 families, were observed to have their barks valued for medicine for diverse
diseases. The methods of preparations and utilizations of the plant-based medicine
were simple and easily adoptable by the respondents. The extraction methods utilized
sourcing for barks were mostly predatory and annihilative. Most of the indentified
species were indigenous, uncultivated with poor regenerative abilities thus most of
them were presently not abundant in the study area. Conservation measures that could
enhance their sustainability were prescribed.
ORIGINAL RESEARCH ARTICLE | Feb. 15, 2018
A Comparative Study on Growth of Dunaliella Salina Treated with Ethyl Methane Sulfonate (EMS)
Chanchal Lata, Gajendra Pal Singh
Page no 121-126 |
10.21276/haya.2018.3.2.4
Micro algae Dunaliella salina is isolated from Sambhar Salt Lake, which
has very high amount of carotenoids. We studied growth of D. salina in various
concentration of EMS through rapid addition and having 1 hour treatment in dark to
find out effective and efficient dose of EMS for D. salina. Studies revealed the
effectiveness and efficiency decreased with increase in EMS concentration. Lower
concentration of EMS was more effective and efficient for promoting growth.
Maximum growth was found in control as compare to EMS treated cultures. The
concentration of EMS chosen reduced the rate of growth, cell movement and cell
survival as compare to control. This reduction increased with increasing
concentration of EMS. Thus the mobility and survival rate of D. salina was
negatively related to the concentration of EMS. Rapid addition of EMS exhibited
high growth in comparison to 1hour treatment of EMS in dark.
ORIGINAL RESEARCH ARTICLE | Feb. 15, 2018
Chemical Composition and Insecticidal Activity of Mentha rotundifolia L. Essential Oil against Sitophilus granarius
Leblalta Amina, Harzallah Daoud, Demirtas Ibrahim, Karakoç Ömer Cem, Gül Fatih
Page no 127-133 |
10.21276/haya.2018.3.2.5
In order to contribute to the development of an integrated pest
management strategy for stored grains, the insecticidal activity of Mentha
rotundifolia L. essential oil was evaluated against Sitophilus granarius, wheat weevil.
Insects and toxicity tests were carried out under laboratory conditions at a
temperature of 27 ± 2 ° C and a relative humidity of 60 ± 5%, at Çankırı Karatekin
University, Yapraklı Vocational School, Department of Animal and Plant Production.
The essential oil was analyzed by gas chromatography (GC) and gas
chromatography/mass spectrometry (GC/MS) to give 14 compounds in the essential
oil of M. rotundifolia. The major component of the oil was identified as 3-
Cyclopenten-1-one, 2-hydroxy-3-(3-methyl-2-butenyl)- (89,09%). M. rotundifolia
essential oil has remarkable insecticidal properties. Highest contact toxicity (100%)
of the oil was observed against S. granarius (1µL/insect application of 4% (v/v) oil
solution in acetone, after 24 h). Highest fumigant toxicity (100%) was observed for S.
granarius (10µL/10mL container application of 1% (v/v) oil solution in acetone, after
24 h). The set of knowledge elements obtained should help guide strategies for the
integrated protection of the grain quality during the storage of local cereals
production.
ORIGINAL RESEARCH ARTICLE | Feb. 20, 2018
Dye Yielding Woody Plants along the National Highway from Parwanoo to Kaurik in Himachal Pradesh
Priya Kumari, Dr. Mukesh Kumar Seth, Rakhi Gagotia
Page no 134-139 |
10.21276/haya.2018.3.2.6
Himachal Pradesh experience diverse climatic condition due to wide
variation in altitude ranging from 350 m in the southern tract to over 6500 m in the
main Himalaya and in the area lying in it6. National highway from Parwanoo to
Kaurik is very rich in biodiversity as well as forest cover along side. Along the side
of this highway have number of dye yielding woody plants which produce different
colours. Locally peoples use these plants for different purposes. Total 51 species
belonging to 39 genera and 30 families have been recorded and identified with
scientific name, family name, common name, part used and colour. Rosaceae is found
to be dominant with 9 species followed by Leguminosae with 6 species,
Berberidaceae having 4 spp., Euphorbiaceae having 3 spp., Anacardiaceae and
Vitaceae with 2 species each and rest of family having one species each.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Seroprevalence and Risk Factors of Toxoplasmosis among Pregnant Women in the Region of Setif, Algeria
Kamel Sofia, Okba Faiza, Rihane Rania, Bounechada Mustapha, Bouharati Khaoula, Khenchouche Abdelhalim, Tedjar Lamri, Tamerni Omar
Page no 140-143 |
10.21276/haya.2018.3.2.7
The aim of this study was to evaluate the seroprevalence of toxoplasmosis
infection in pregnant women in Setif region and to identify the main risk factors
involved. The different parameters were collected by direct interviews and using
standardized ELIFA technique for detecting the anti-toxoplasmic antibodies IgG /
IgM and determining the immunological status of this sample. Our results showed a
general lack of Toxoplasmosis awareness, a prevalence of 58% of seronegative
pregnant women and 42% of seropositive women they may have risks of
seroconversion as the case observed in our study. The statistical analysis confirmed
that the consumption of undercooked meat is the main factor of contamination. This
study has the advantage of setting up a serological monitoring of toxoplasmosis
according to a legal framework with the aim of detecting and servile seronegative
women, hence the importance of education and information in terms of prevention.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Assessment of In-Vitro Anti-Arthritic Potential of Methanolic Leaf Extract of Ricinus communis
Abdul Mukit Barbhuiya, M. Ramya Sri, Ch. Goutami, R. Sai Sindhu
Page no 144-147 |
10.21276/haya.2018.3.2.8
The use of plants for the treatment of various diseases was recorded in the
history. Many conventional medicines originate from plant sources: many decades
ago, most of the few effective drugs were plant-based. Examples include quinine
(from cinchona bark), digoxin (from foxglove), morphine (from the opium poppy)
and aspirin (from willow bark). The discovery and development of new drugs from
plant sources continues, with drug companies engaged in large-scale pharmacologic
screening of herbs. Arthritis is a condition typically characterized by pain, aching,
stiffness and swelling in and around one or more joints. Rheumatoid arthritis (RA) is
a disorder in which the body's own immune system starts to attack body tissues.
Ricinus communis, the castorbean or castor-oil-plant is a species of flowering plant in
the spurge family, Euphorbiaceae. Many authors have reported that the plant has
promised Antioxidant activity, Antinociceptive activity, Antiulcer activity,
Antiasthmatic activity, Anti-fertility activity, Antihistaminic Activity etc cited in
literature review. The present study is aimed to evaluate the methanolic leaf extract of
Ricinus communis for acclaimed anti-arthritic activity by using protein denaturation
method. Diclofenac sodium is used as a standard drug. From the results of the present
study it can be stated that the methanolic leaf extract of Ricinus communis at two
different concentrations (250mcg/ml & 500mcg/ml) is capable of inhibiting the
denaturation of protein in rheumatic disease.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Evaluation of In-Vitro Anti-Urolithiatic Activity of Methanolic Leaf Extract of Ricinus communis
Abdul Mukit Barbhuiya, M. Ramya Sri, Ch. Goutami, R. Sai Sindhu
Page no 148-151 |
10.21276/haya.2018.3.2.9
In the current scenario herbal medicines became the main system for the
treatment of many diseases. Because of the advancement in science and technology a
huge number of plants were discovered having therapeutic capabilities to treat many
deadly diseases and have gain importance and acceptance within the medical
community. Among various diseases urolithiasis has been a common problem from
various centuries. For the treatment of urolithiasis many synthetic drugs are available
in the market but because of their side effects herbal remedies are widely accepted.
Our present study aimed to evaluate the therapeutic potential to treat urolithiasis
(kidney stones) where cystone was used as a standard drug. The present study
revealed that the leaves of Ricinus communis possess mild antiurolithiatic activity
which might be due to the presence of various phytochemical constituents such as
flavonoids, alkaloids, triterpenes etc.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Formulation and Evaluation of Sustained Release Tablet of Etodolac
M. P. Shirbhate, M. J. Chavan
Page no 152-164 |
10.21276/haya.2018.3.2.10
Sustained release formulations are becoming more popular now days for
the delivery of non-steroidal anti-inflammatory drugs (NSAIDs) because of their
ability to maintain therapeutic effective drug concentration for prolonged duration
with low dosing frequency and side effects associated with NSAIDs. The present
study was attempted to develop Sustained release tablets of a model NSAID drug,
Etodolac. Etodolac Sustained release tablets were prepared by Gellan Gum (A, mg),
Sodium CMC (B, mg), Xyloglucan (C, ml), Xanthan Gum (D, ml), MCC (E, ml),
Talc (F, rpm), Orange flavour (G, rpm), Aspartame (H, rpm), Magnesium stearate (I,
rpm). The granules were evaluated for flow properties by evaluating bulk density,
tapped density, Carr’s index, Hausner’s ratio and angle of repose. The tablets were
evaluated for drug polymer compatibility study by FTIR, diameter, weight variation
test, hardness, friability, disintegration test, SEM, Swelling Index, In vitro drug
release, release kinetics, stability studies and Plackett-Burman Experimental Design
was also applied to find the optimized formulation. The FTIR study revealed that no
such interactions being taking place in between drug and polymers. The flow
property of granules of all tablet batches was found to be good. All the tablet
formulations had good tablet physiochemical properties. The swelling of the tablets
was also found optimum. From the results of in-vitro study, it was concluded that
Etodolac Sustained release tablet provided most sustained release of Etodolac over
extended period of time with aid of greater stability.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Herbaceous Fodder Plants along the National Highway from Parwanoo to Kaurik in Himachal Pradesh, India
Rakhi Gagotia, Dr. Mukesh Kumar Seth, Priya Kumari
Page no 209-213 |
10.21276/haya.2018.3.2.16
A survey of herbaceous fodder plants along the national highway from
Parwanoo to Kaurik was carried out. Mostly along the national highway rural areas
were situated and they used local flora for their cattle or livestock. Some of the
herbaceous plant species used as fodder among the communities of these areas. In
Present paper, 53 species belonging to 17 families and 44 genera were recorded and
identified. The families with higher number of species were Poaceae with 29 species,
Leguminosae with 5 species, Brassicaceae and Cyperaceae with 2 species,
Acanthaceae, Begoniaceae, Caryophyllaceae, Commelinaceae, Compositae,
Convolvulaceae, Cucurbitaceae, Datiscaceae, Oxalidaceae, Pedaliaceae,
Polygonaceae, Solanaceae, Urticaceae with 1 species each. The genera represented by
the higher number of species Setaria (3 species), Chrysopogon, Cyperus, Oplismenus,
Paspalum, Saccharum, Trifolium (2 species) and rest of the genera comprising only
one species.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Decomposition Pattern of Bio-Slurry in two Contrasting Soils of Bangladesh
Md. Mamunur Rashid, Majharul Islam, Md. Zakaria Ibne Baki, Md. Maksudul Haque, Dr. M. Mazibur Rahman, Dr. Abdul Kader
Page no 165-175 |
10.21276/haya.2018.3.2.11
Bio-slurry has potential value as good quality organic fertilizer and an
agent of soil carbon sequestration through build up of soil organic matter level. Cowdung and poultry manure had the higher organic carbon content as well as higher
nutrient concentration compared to Cow-dung and poultry bio-slurry. Nutrient
concentration particularly N, P and S in CD and CB was found lower than the PM
and PB. The highest carbon mineralization in Balina soil was found in poultry
manure amended soil followed by cow dung, poultry bio-slurry and the lowest in cow
dung bio-slurry. Carbon mineralization of manures in Noadda soil also followed the
similar trend like Balina soil. However, Carbon mineralization of poultry manure and
poultry bio-slurry was lower and cow dung and cow dung bio-slurry was higher in
Noadda compared to Balina soil. Thus, decomposition of manure does not depend
only on the quality of soil rather both the quality of manure and soil. Decomposition
of manure was found slightly higher (around 5%) in Balina soil compared to Noadda
soil when averaged over four manures. Among the manure, around three to four times
less amount of CO2 was evolved during the decomposition of cow-dung and poultry
bio-slurry as compared to cow-dung and poultry manure. Thus, this study indicate
that soil application of bio-slurry had high potential in the mitigation of the
greenhouse effect as well as short-term benefits in terms of improving soil organic
matter stock, as compared to manure.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Application of Myristica fragrans feed in Poecilia latipinna as an effective antibacterial agent and colour enhancer
Divya MS, Dr. Sreeja J
Page no 176-179 |
10.21276/haya.2018.3.2.12
Myristica fragrans seed (Nutmeg) famed as the prominent fatty seed of
medicinal and therapeutic quality came from the Spice island of Indonesia. It is
endowed with phytochemicals, minerals and vitamins impart medicinal values. In
captive conditions the ornamental fishes with deprived nutritional feed subjected to
diseases and faded colour produces economic loss in their trade operations can be
tackled by effective administration of plant products. In this perspective, a
comparative study made in Poecilia latipinna (yellow molly)by providing
(commercial feed) CF and TD1 (Myristica fragrans) for a duration of 60 days. The in
vivo antibacterial efficacy of feed in tissues(gut & gill) of the specimen along with
water samples from aquaria revealed positive result for TD1.The colour enhancing
ability in yellow molly yielded best result higher for TD1 considered to CF. The total
bacterial load in the water, gut as well as the gill of CF fed group was 75x104
CFU/ml, 45x104 CFU/ml& 23x104 CFU/ml while for TD1 it was 14x104 CFU/ml,
9x104 CFU/ml, 7x104CFU/ml. The quantitative estimation of carotenoids estimated
was 6.32µµg/g wet weight in control and 12.12µg/g wet weight for TD1.From the
present investigation arrived at the conclusion that TD1 provided better anti-bacterial
activity than CF (commercial feed) not only prevent disease outbreak but also
produce vibrant skin colour.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Endocrine Changes Associated with Ovarian Activity in Montbéliarde Cows Raised Under Semi-arid Condition in Eastern of Algeria
Nabila Kara, Mustapha Bounechada, Houssam Chebal, Khaled Maouche, El-eulmi Lounis, Aid Meratla, Badredine Bouchama, Belkacem Chawki Chaib
Page no 180-187 |
10.21276/haya.2018.3.2.13
The objective of the current study was to investigate changing profile of
reproduction hormone (Progesterone and Estradiol) BCS and parity in relation to the
resumption of ovarian cyclicity postpartum in cows of European origin under semiarid conditions of Algeria. To measure plasma progesterone (P4) and estradiol 17-β
(E2), blood samples were collected from 21 Montbéliarde dairy cows at 30, 40 and 50
d postpartum (dPP) respectively. Body condition score (BCS) was taken before and
after calving. Cows were grouped based on progesterone concentration (resumption
of ovarian cyclicity, ≥1 ng/mL) at 30, 40, and 50 dPP into a non ovarian activity
(NOA) group (n = 6) and ovarian activity (OA) group (n = 15). P4 concentration was
higher in the OA group than in the NOA group and statistically differed at 50 dPP (p
= 0.04). E2 was higher in the OA group than a NOA group at d 30 (p = 0, 01) and d
40 (p = 0, 03). Despite superiority of BCS peri and postpartum but no significant
differences were detected between the two groups. There was no significant
difference between parity and ovarian resumption groups. Two groups were formed
based on differences in onset of postpartum resumption of ovarian activity. OA (n
=15/21 or 71.42 %) showed first ovulation between 30 d and 50 d after parturition.
NOA (n = 6/21 or 28.57 %) manifested a first ovulation > 50 d in milk.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Diversity of Genus Ganoderma in Nagaon District, Assam
Ratul Kumar Nath, T. C. Sarma
Page no 188-195 |
10.21276/haya.2018.3.2.14
Nagaon district of Assam is located in the flood plains of the river
Brahmaputra. The physiographic features of Nagaon District makes it ideal home for
the growth of different types of economic plants. But these plants are found to be
decayed by the association of some macro fungi. Among these macro fungi, different
Ganoderma species were found to be associated with the different timber yielding
plants and palm species. Ganoderma with great diversity produce large fruitification
of different size, shape and colour. During the investigation 7 seven Ganoderma
species were collected from 10 different Revenue Circles of Nagaon district. This
work revealed that there are great diversity of Ganoderma species in Nagaon district,
Assam. Some Ganoderma species have medicinal properties.
ORIGINAL RESEARCH ARTICLE | Feb. 28, 2018
Deal or no Deal: Reflections on the Paris Agreement on Climate Change
Dr. Shadi A. Alshdaifat
Page no 196-208 |
10.21276/haya.2018.3.2.15
In December of 2015, the 21st Conference of the Parties to the United
Nations Framework Convention on Climate Change (UNFCC COP21) took place in
Paris. UNFCC is an international environmental agreement on climate change, of
which there are 195 States Parties. The U.N. Intergovernmental Panel on Climate
Change (IPCC) has warned of the consequences of failing to limit global temperature
rises to at least 2 degrees Celsius (above pre-industrial times), highlighting that the
impacts would pose a threat to humanity and could lead to irreversible climate
change. The meeting in Paris was hailed as a make-or-break opportunity to secure an
international agreement on approaches to tacking climate change, a commitment to a
longer-term goal of near zero net emissions in the second half of the 21st century, and
supporting a transition to a clean economy and low carbon society. This paper
discusses the issues and solutions of climate change, development and critical views,
the UAE environmental regulation, company's environmental responsibility, and the
future of the Paris Convention on Climate Change.