Indiscriminate usage of Cassia occidentalis in treating many diseases has been locally practiced, the need for ascertaining the epicacy of the extract in management of liver and kidney damage become imperative. This research investigate the effects of aqueous root extracts of Cassia occidentalis on some liver and kidney parameters in acetaminophen induced liver and kidney toxicity. A total of thirty five rats were used for the research, in phase I, ten rats were used to confirm the inducement of hepato-renal toxicity by acetaminophen. They were grouped into two of five rats each, group I serve as normal control, group two serves as test control and administered with 750mg/kg body weight of acetaminophen. The animals were euthanized after 24 hours of acetaminophen administration and liver function indices (ALT, AST, total protein, Albumin total and direct Bilirubin) and kidney function indices (urea and creatinine) were assayed. In phase II, twenty five rats were grouped into five groups (GI – GV) of five rats each. G I served as normal control, GII served as test control, GIII, GIV and GV were induced with hepato-renal toxicity and administered with the extract at a dose of 50mg/kg, 100mg/kg and 150mg/kg body weight per day for two weeks. A significant increase (p<0.05) in both liver and kidney function indices was observed in test control group compared to normal control in both phases. Administration of the extract lead a significant decrease (p<0.05) in liver parameters in a dose dependent manner compared to test control. Contrary to this however, a slight decrease in serum urea and creatinine was observed in the extract administered groups. The observed hepatocurative ability of the plant may not be unconnected with the presence of various phytochemicals in the plant.

West Nile virus (WNV) is a small, enveloped, mosquito transmitted virus with a positive sense, 11 kb RNA genome. It was first isolated in 1937 from a woman with an undiagnosed febrile illness in the West Nile district of Uganda, WNV is currently the most widely distributed arbovirus in the world, occurring in all continents except Antarctica, there are no clinically approved vaccines or antiviral drugs, thus, the aim of this study is to analyze envelope glycoprotein variants using in silico approaches looking for conservancy, which is further studied to predict all potential epitopes that can be used after in vitro and in vivo confirmation as a therapeutic peptide vaccine. Total of 149envelope glycoprotein variants retrieved from NCBI database were introduced to IEDB conservancy tool, to select the highly conserved regions for epitopes prediction. By means of IEDB analysis resource B and T cell epitopes were predicted and population coverage was calculated. Three epitopes were proposed for international therapeutic peptide vaccine for B cell (DLSTKAACPTMGEAHNDKRADPAFV, YGEVTVDCEPRSGIDTN and PVEFSSNTVKL). Regarding T cell, RSYCYLATV epitope was highly recommended as therapeutic peptide vaccine to interact with MHC class I along with five other epitopes that showed good population coverage against whole world population. Two epitopes showed high affinity to interact with MHC class II alleles ( IKYEVAIFV and WFMDLNLPW ). Epitopes defined by methods such as the one described here are likely to contribute substantially to the development of new in vivo and in vitro research ,diagnostic and vaccines for WNV and other emerging infectious diseases.