The leading environmental risk factor for disease and premature death is pollution, with plastic and dye pollutants being the most common in developing countries. The global textile industry contributes to pollution by releasing contaminated wastewater into water bodies, leading to a decline in water quality. Plastic pollution is a widespread issue affecting various environments, emphasizing the urgent need for a global response to combat the adverse effects of pollution on human health and the environment. Anoxybacillus sp. PDR2 is a soil bacterium possessing natural competence. By nature, it is a thermophile, capable of biodegrading industrial dyes. Pseudomonas sp. B10, Gram-negative bacteria, is a strain capable of degrading Polyethylene Terephthalate (PET) plastic. To detect the genome-level mutations, comparative genomic analysis was performed using a free and open source software, Galaxy. Using five different variant callers (Samtools, Varscan, Freebayes, Sniffles, Ivar), mutations were detected at various loci resulting in the modifications of the genes. The primary goal of this investigation was to perform a comparative analysis of the whole genome sequencing of two bacterial species, along with their reference strains. The purpose was to identify potential solutions for the degradation of plastics and industrial dyes. By examining the genetic composition of these bacteria, this analysis had provided valuable insights into the genetic makeup of these bacteria and their ability to break down PETs and dyes.

Background: Because GI cancer is the most common type of cancer seen every day in Sudan's clinical practice, research into this illness is critical. Our study sought to determine its epidemiological characteristics. Methodology: This is a retrospective descriptive study that includes all patients who presented with GIT tumors between January 2019 and July 2024 at the EL-Obeid Histopathology Center in North Kordofan. Results: This study included 127 individuals with GIT cancer, with 52% being male and 48% being female. Their ages varied from 20 to 98 years, with an average of 57.9±15. The age group (51–60) had the highest proportion of research subjects, with 66.7% being men, followed by the age group (61–70), with 54% being female. The most common kinds of GIT cancer were esophagus, colon, stomach, rectum, liver, small bowel, bowel rectosegmoid, and appendix, accounting for 35%, 25%, 16%, 11%, 8%, 3%, 1%, and 1%, respectively. SCC was the most common histological diagnosis, accounting for 96.6% in the esophagus, followed by AC (37.5%), 34.4%, and 21.9% in the colon, stomach, and rectum, respectively. Hepatocellular carcinoma is the diagnosis for 100% of liver cancers. AC accounted for 35.6% of all histological diagnoses across age groups, with the majority occurring in the 51-60 age range, followed by SCC at 27.6%. In all cases, 40% of others' histological grades are highly defined, with the majority developing between the ages of 61 and 70. Conclusion: The most prevalent cancers in Western Sudan were esophageal, colonic, stomach, rectal, and liver. Many incidents occur when people are over the age of 50, and they affect both men and women.