Objectives: The aim of this study was to investigate the concentration of hemoglobin, ferritin, albumin, total protein and globulin in pregnant Sudanese women to assess the presence of anemia and hypoproteinemia in the second and third trimester. Materials and method: A total of 100 pregnant women were attended at Almadina Arab Hospital in Gezira state, Sudan in the period from December 2017 to February 2018. Their ages ranged from 19 to 37 years old, and they were divided into two groups: second trimester and third trimester. Venous blood samples were drawn from each participant to determine hemoglobin, ferritin, albumin, globulin, and total protein levels using a Spectrophotometric method by an automated analyzer (Biosystem A25), and globulin was determined by calculation. The statistical package for social science (SPSS) software version 20 was used to analyze data. Result: In both the second and third trimesters, the mean concentration of measured biochemical parameters were decreased, with no significant difference between the two groups however, the means of ferritin levels were normal. Hemoglobin mean levels were (10.784±1.143 and 10.968±1.570), total protein mean levels were (6.632±0.701 and 6.644±0.631), albumin mean levels were (3.204±0.423 and 3.254±0.420), and globulin mean levels were (3.444±0.661 and 3.390±0.611) in the second and third trimesters, respectively. The mean levels of ferritin were (36.900±1.458 and 39.300±1.591) in the second and third trimesters, respectively. Conclusion: Pregnant Sudanese women in their second and third trimesters were found to have anemia and hypoprotienemia.

The risk of osteoporosis is higher in postmenopausal women and nutritional factors reported playing an important role in the etiology of low bone mineral density (BMD). Some studies claimed the involvement of vitamin B12 in the quality of bone structure in humans, however, results are not conclusive. The objective of this study was to explore the relationship between BMD and vitamin B12 levels in postmenopausal women. In this cross-sectional study, BMD and blood samples from 77 postmenopausal women (age > 45 years) were studied. BMD and T-scores of the study subjects were determined at the femoral neck and lumbar spines by Dual Energy X-ray Absorptiometry (DEXA). Serum VitB12 was measured by enzyme-linked immunosorbent assay. The mean±SD age of the postmenopausal women was 56.4±7.9 years. Bone mineral densities (g/cm2) were 0.80±0.16, 0.76±0.18, 0.74±0.14 and T-scores were -2.21±1.45, -1.41±1.22, -1.53±1.20 respectively at the lumbar spine, right femoral neck and left femoral neck. The mean±SD of vitB12 was 245.9±274.3 pg/mL. On multiple regression analysis, β values for log(VitB12) with BMD were 0.119 (p = 0.018), 0.085 (p = 0.140), 0.011 (p = 0.012) and with T-score were 1.028 (p = 0.022), 0.698 (p = 0.064), 0.940 (p = 0.015) at the lumbar spine and right femoral neck and left femoral neck respectively. In conclusion, vitamin B12 is found to be positively associated with bone mineral density and T-score at the lumbar spine and left femoral neck but not at the right femoral neck in postmenopausal women.