Black humor emerged as a distinctive fictional genre in the United States in the 1960s. In the creative process, novelists tend to emphasize the unique charm of this genre through the portrayal of anti-heroes. Thomas Pynchon is a forerunner in American black humor novels during the 1960s and 1970s, and his masterpiece Gravity’s Rainbow is an outstanding example of this genre. This article focuses on two representative anti-heroes in Gravity’s Rainbow, Tyrone Slothrop and Roger Mexico. Through a thorough analysis of Pynchon’s humorous descriptions of the difficulties faced by these two characters, the black humor characteristics of this novel are interpreted, and readers can gain a deeper understanding of Pynchon’s creative style and the essence and appeal of black humor.