As a follow-up to an earlier report on the antihyperlipidaemic activity guided fractionation study on the chloroform extract from the sclerotia of the edible mushroom Pleurotus tuber regium, this present study is reporting the isolation of a sesquiterpene enol carboxylic from the active chromatography fraction of the chloroform extract of this edible mushroom. The purity of the compound was confirmed from TLC and Melting point determination. The chemical structure was elucidated using spectroscopic techniques (Mass spectrometry, 1&2D Nuclear magnetic resonance and Infra red spectroscopy). The isolated compound coded as compound P, exhibited molecular mass of 256 with molecular formular of C14H24O4 and from the 1 &2D NMR and IR analyses, the compound P was identified as a sesquiterpene enol carboxylic acid with a sytemic name of 2,3-dihydroxy-10-propylcyclodec-2-ene-1-carboxylic acid.

The Schiff base ligand has been synthesized by the reaction of ethanolic solution of benzaldehyde and 2,4-dinitrophenylhydrazine. The corresponding metal complexes were obtained by refluxing the chlorides of Co(II) and Ni(II) with the prepared Schiff base in an ethanolic medium. The Schiff base and its metal complexes were established and analyzed by FT-IR, solubility test, melting point/decomposition temperature and conductivity measurement. The melting point of the Schiff base was found to be 157C and decomposition temperature of Co(II) and Ni(II) complexes were found to be 167°C and 174°C respectively. Molar conductance values of Co(II) and Ni(II) complexes are 1.31 and 2.04 µS/cm respectively. The solubility test carried out showed that the complexes are soluble in most organic solvents and insoluble in water. The antibacterial activity test of the ligand and metal complexes showed that the metal complexes were found to be active against the organism tested while the ligand was inactive.