ORIGINAL RESEARCH ARTICLE | May 18, 2022
Comparison of Antioxidant, Flavonoid and Polyphenol Content of Three Selected Solanaceae Genera from Kigezi, Southwest Uganda
Mukasa-Tebandeke, I. Z, Karume, I, Ssebuwufu, J, Wasajja, H. Z, Nankinga, R. M
Page no 40-60
Solanaceae is family of plants widely used in food, sauce and herbal medicine because its members are rich in carbohydrates, proteins, fats, vitamins, mineral salts and other trace and essential nutrients like phenols and flavonoids that retard degenerative diseases and stress. Knowledge on purposeful use of solanaceae in food and medicine in Uganda is low. Aqueous extracts of dry leaves of three selected solanum genera growing in Kabale were compared for their polyphenol and flavonoid contents and antioxidant properties. Total polyphenol (TPC) and total flavonoid (TFC) contents were determined by the Folin-Ciocalteu and aluminium chloride colorimetric methods respectively. Antioxidant properties and radical scavenging were determined using DPPH, hydrogen peroxide, thiocyanate-iron (III) complex and iron (II)/(III)-linoleic acid systems. The polyphenol content of dry leaves of S. anguivi was 1750 ± 0.70, that for S. macrocarpon was 104 ± 0.45 and S. nigrum was 97.80 ± 0.15 GAE/g yet the flavonoid content was 7.40 ± 0.30, 35.00 ± 0.60 and 16.40 ± 0.40 mg/QE/g for S. anguivi, S. macrocarpon and S. nigrum respectively. The DPPH scavenging at IC50 were 7.80 ± 0.25; 45.60 ± 0.30 and 42.90 ± 0.20 respectively yet hydrogen peroxide scavenging at IC50 stood at 6.89 ± 0.15; 27.00 ± 0.35 and 17 .90 ± 0.20 μg/mL in the respective order for S. anguivi, S. macrocarpon and S. nigrum. The available data suggests the plants are very good food supplements of high nutritive and chemotherapeutic values. However, there is need to perform in vivo and vitro experiments to deduce their efficacy on mammals.
ORIGINAL RESEARCH ARTICLE | May 26, 2022
Phytochemical Investigation and Determination of Antibacterial Activities of the Fruit and Leaf Crude Extract of Ficus palmata
Shashe Kassaw, Addisu Tamir, Biruk Bezabeh Yimam
Page no 61-66
To cure ailments, the global population makes medicines from plants. Ficus palmata can be used to cure a variety of ailments. The purpose of this study was to examine the phytochemical content and antibacterial activity of methanol extract from Ficus palmata fruit and leaf crude extract. Maceration was used to make the methanol extracts, which were subsequently fractionated. The crude extracts and fractions were subjected to standard phytochemical screening assays. Phytochemical screening revealed the presence of flavonoids, alkaloids, saponins, tannins, polyphenols, anthraquinones, steroids, coumarins, and terpenoids in the crude methanol fruit extract. Phytochemical screening revealed the presence of flavonoids, alkaloids, tannins, polyphenols, anthraquinones, steroids, and terpenoids in the extract. The determination of trace metals in leaves and fruit samples using a flame atomic absorption spectrophotometer revealed that the fruits contained relatively high quantities of iron and other nutritionally essential elements, such as magnesium, calcium, and copper, whereas the leaves contain a high level of calcium, magnesium, and iron, as well as a small amount of copper.