One of the most important parameter in elongation of cotton fibre is the biosynthesis of ethylene. EIN3 and EIL1 (Ethylene Insensitive3/Ethylene Insensitive 3) are the main transcriptional factors that are specifically playing an important role in signal transduction in cotton. Expression analysis of the EIN3/EIL1 genes involved in development of cotton fibre investigated out through RT-qPCR. Further, STRINGTIE is a tool that is use for measuring the different levels of expression. ClustalW method is a method that is used to determine the sequences of amino acids of EIN3/EIL1 genes by aligning by using the sequences. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of the EIN3/EIL1 genes is also bioinformatics tools that is functionally use for classification and characterization of genes at functional level with using the principles of functional enrichment analysis. Biological database such as STRING is designed for to construct the specific PPI networks of EIN3/EIL1 due to known PPIs and analysis to check interactions among protein. Bioinformatics tool such as BLASTP use for identification of transcription factors of EIN3/EIL1genes. Biological database such as Plant CARE is especially used to analyze the cis-elements in specific sequences of promoters of EIN3/EIL1 that are especially involved in transcription of genes.

Green tea is one of the most popular drinks consumed worldwide. Produced mainly in Asian countries from the leaves of the Camellia sinensis plant, the potential health benefits have been widely studied. Recently, researchers have studied the ability of green tea to eradicate infectious agents and the ability to actually prevent infections. The important components in green tea that show antimicrobial properties are the catechins. The four main catechins that occur in green tea are (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC), and (-)-epigallocatechin-3-gallate (EGCG). Of these catechins, EGCG and EGC are found in the highest amounts in green tea and has been the subject of most of the studies. These catechins have been shown to demonstrate a variety of antimicrobial properties, both to organisms affected and in mechanisms used. Consumption of green tea has been shown to distribute these compounds and/or their metabolites throughout the body, which allows for not only the possibility of treatment of infections but also the prevention of infections.

There are thirteen vitamins in humans: four fat soluble (A, D, E, and K) and nine water soluble (vitamin B complex and vitamin C). Water-soluble vitamins dissolve rapidly in water and are usually easily excreted by the body. Because they are not processed too soon, a steady intake is important. B vitamin supplements available for each vitamin: B1: thiamine, B2: riboflavin, B3: niacin, etc. Niacin, pantothenic acid, biotin and folate are recognized by name rather than by quantity. B vitamins are usually used in energy drinks and many are advertised with high levels of B vitamins boasting that they can 'go through the day and do not feel nervous or anxious. B vitamins are primarily absorbed in foods such as pork, fish and liver. Healthy vitamin B sources include vegetables (pulses or beans), whole grains, rice, bananas, chilli pepper, tempeh, brewer's yeast and molasses. While beverage yeast has been used to produce beer, its bioavailability varies from low to adverse, as drinking ethanol hinders the absorption of thiamine (B1), riboflavin (B2), niacin (B3), biotin (B7) and folic acid (B9). Vitamin C is needed for tissue growth and repair in all areas of the body. It is used as an essential protein for the development, repair and maintenance of cartilage, bones and teeth and assists in the absorption of iron.

Objectives: The aim of the present study was to investigate the relationship between nutrition and sunlight exposure on BMD levels in different age group between 30 to 75 years old subjects and to assess the relationship between age, sex and BMD in general population. Design: An observational cross sectional study of randomly selected in kanchipuram district aged from 30–75 years. Materials and methods: The study was conducted in the department of biochemistry, Meenakshi Medical College Hospital and Research Institute, Kanchipuram .Under this cross-sectional comparative study. 80 subjects of both sexes were subjected to the study. And subjects are divided into group 1(Age Group between 30-45), group 2 (Age Group between 46-60), and group 3 (Age Group between 61-75). Nutrition status and exposure to sunlight was collected by questionaries. Results: 80 adults between 30 to 75 years of both sexes were subjected to this study. People in the age group of 30-45 years were found to be more osteopenic particularly very high in females (males -0.97±1.2 and females -1.3±1.45) and people in the age group 61 to 75 years, were found to be osteoporotic (males -1.43±1.3 and females -2.08±0.95). Based on nutrition, 15 people of both sexes were selected who are vegetarian (-0.96 ± 0.6), 22 people were selected who take non-veg three times in a week (1.29 ± 0.92), and 28 people were selected who take non veg more than 3 times in a week (1.3 ± 0.77) and 15 people were selected who take both veg and non-veg in a week (1.38 ± 0.77). The results indicate that vegetarian taking people were got low BMD levels when compared to other groups. BMD level significantly increased in people exposed to sunlight for more than 10 minutes when compared to less exposed to sunlight. Conclusion: This study confirms that people of younger age group (30-45 years) are found to be more osteopenic when compared to the older age group (46-60 years) due to of less exposure in sunlight and low intake of vitamin D and calcium diets. Highlight of this study is “Osteopenia is more prevalent in the younger age group which necessitates earlier intervention”