Background: There is evidence that support the use of high sensitive C-reactive protein (hs-CRP) to monitor cardiovascular risk in diabetic and non diabetic individuals. This study aims to assess hs-CRP level in Sudanese with type 2 diabetes mellitus (D.M) and obese individuals; as a risk factor for cardiovascular diseases. Materials and methods: This cross-sectional study was conducted in Khartoum-Sudan from April to December 2018, 50 diagnosed Sudanese patients with diabetes mellitus attending Alarbaeen hospital in Khartoum state and 50 obese Sudanese individuals were enrolled in this study. Blood samples were analyzed for cholesterol and hs-CRP levels using Mindray BS-200 automated analyzer. Results: There were no significant differences in hs-CRP and total cholesterol levels in type 2 D.M when compared with obese. Both diabetic patients and obese had high levels of hs-CRP and total cholesterol. hs-CRP and total cholesterol levels revealed no significant difference when male compared with female in type 2 D.M as well as in obese. The majority of the study participants were found to have hs-CRP level more than 3 mg/L, which indicate a high risk to have cardiovascular diseases according to American Heart Association. There was no correlation between hs-CRP levels and total cholesterol levels in the study subjects. Age showed inverse significant correlation with hs-CRP levels in type 2 D.M and no correlation among obese. In conclusion: The higher levels of hs-CRP with higher levels of total cholesterol may be a predictive factor for developing of CVD in Sudanese with type 2 D.M as well as in obese.

PPAR-γ plays a critical role in glucose homeostasis and is a molecular target of insulin sensitizing drugs called thiazolidinediones which are PPAR-γ ligands. PPAR-γ ligands also upregulate expression of adipocyte glycerol kinase, resulting into improved insulin action in target tissue such as liver and muscle. PPAR-γ ligand also shown to augment glucose disposal in peripheral tissues by increasing expressions of the glucose transporter genes, GLUT-1 and GLUT-4. Majority of the Diabetes Mellitus Type 2 (DM type 2) patients (80%) are obese, but all obese persons do not develop DM type 2. Serum adiponectin level should be determined by level of PPAR-γ activity in adipocytes. Therefore it will be of interest to explore the difference in the level of serum PPAR-γ level between obese subjects with DM-2 and without DM-2, as both the insulin sensitizing factor’s adiponectin, and vistafin are synthesized in response to FFA. PPAR-γ complex receptor activity. Estimation of serum PPAR- γ level alongwith FBG was done in 90 cases, of which 30 were normal healthy subjects, 30 obese without diabetes type 2 and 30 obese with diabetes type 2. The qualitative estimation of PPAR-γ in human serum was done by ELISA method using ELISA kit from E lab science Biotechnology Co.Ltd. In our study we found a significant change in the level of PPAR- γ between the obese with DM-2 and control subject and also between obese without DM-2 and control subject but we did not find any significant difference between obese with DM-2 and obese without DM-2. In our present study we found the Increased Levels of PPAR- γ in the Obese without Diabetes Mellitus and Obese with Diabetes Mellitus. The levels were 8.03ng/ml ±11.16 and 8.04ng/ml±10.94 respectively. Finally, we observed that the level of PPAR- γ is increased with both the groups, i.e. obese without DM-2 and obese with DM-2 in comparison to normal subjects.

Background: Prevalence of Acute Myocardial Infarction (AMI) in younger age is increasing in worldwide and it has been reported that 60% of AMI in young age is due to genetic causes. SNP inPAI-1 4G/5G plays major role and there is a need to authenticate its effectiveness and sensitivity. The aim of this study is to ascertain SNP in PAI-1 (4G/5G) as an independent biochemical marker for the South Indian young AMI patient’s less than 45 yearsand to find its association with other risk factors of AMI. Methodology: This cross sectional study subjects includes40 Patients aged less than 45 years with AMI withtypical chest pain, ST Elevation in ECG and a rise in serum CK-MB, without any other known risk factorsand age and sex matched 40 control. ARMS-PCR was done with separated DNA. Remaining Serum was used to analyze Urea, HDL, CK-MB and other factors. Finally statistical analysisis done using SPSS-20.0. Result: Statistical analysis showed significant variation of SNP inPAI-1(P ≤ 0.001) between patients and controls. Further, positive significant changeswere observed between patients and controls in Serum Urea: P ≤ 0.001, Serum Glucose: P ≤ 0.04, Serum AST: P ≤ 0.001, Serum CK-MB: P ≤ 0. 001. A significant inverse association was observed in SNP of PAI-1 and Serum HDL. Conclusion: This study confirms the independent association between STEMI and the 4G/5G allele polymorphism among South Indian euthenics and an inverse association of 4G/5G polymorphism with Serum HDL-cholesterol levels.

Prevention of diabetes and its associated burden has become a major health issue worldwide. The present study was undertaken to assess the changes in insulin sensitivity and endothelial dysfunction well before the onset of diabetes in population with positive family history for diabetes. The objectives of the study is to estimate the levels of serum Adiponectin, Visfatin, Insulin, HOMA IR and platelet derived microparticles P-selectin levels in offspring of type 2 Diabetes mellitus. The healthy volunteers who are aged between 18- 22 years of either sex, were selected based on their family history of diabetes. The study showed a significant decrease in serum Adiponectin and increase in serum Visfatin, Insulin, HOMA IR & P-selectin levels in the offspring of type 2diabetes. The Adiponectin showed a negative correlation with Visfatin, P selectin, Insulin & HOMA IR. Genetic predisposition for diabetes may influence adipocytokine levels which might play a key role in developing diabetes in near future.

Healthcare-associated infections (HCAI) are a major setback for any organization. The most important mechanism of spread of these HCAI is via the contaminated hands of the healthcare givers that is doctors, nurses, other staff or relatives/friends of the patients. Contaminated environmental surfaces are another important reservoir for the spread of these infections. However, they are often under recognized. HCA is one of the most important public health problems in most countries of the world. In any healthcare setup basic infection control, measures can reduce the rates of healthcare-associated infections, which help to reduce the length of stay, nosocomial infection and cost. It is important to implement the quality system which can be executed and monitored by using compliance measures in the routine process of the hospital in order to improve the standard of patient health care. The quality of hospital infection control program is a reflection of the overall standard care provided by the institution. The current study is aimed to help in assessing the pre-existing knowledge regarding infection control practices among doctors and nurses. In our study, both doctors and nurses had a fair knowledge about the spread of nosocomial infections, practised safe patient-care protocols, and had positive attitudes. Still, some gaps have been identified in their knowledge and practice of infection control. It is suggested that an effective training sessions should be organized at the time of hire and in-service periodically which might help to decrease the healthcare associated infections.