Hepatocellular carcinoma is considered one of the most threats to human health and is considered a fatal threat globally. Hepatocellular carcinoma is still a health challenge, and its incidence is growing worldwide. The role of non-coding miR-141 RNA in Hepatocellular carcinoma needs more investigations. Micro-RNA consists of 18-22 nucleotides. The role of miR-141 in hepatocellular carcinoma needs many investigations. The expression of miR-141 was elucidated in different cell lines including HepG2, HuH7, and the normal cell lines. The cell survival rate was detected in the case of untreated cells, control cells, and transfection by overexpression vector for miR-141, and anti-miR-141 transfection in the HepG2 cell line. The survival rate was at its highest level in the case of overexpressed miR-141 while it showed the least survival rate at anti-miR-141 transfection. Additionally, the effect of miR-141 was tested on both the IL-6 as an inflammatory cytokine and on TNF-α as well. In conclusion, miR-141 plays a pivotal role in HCC carcinogenesis.

Canola is an important crop with low levels of fatty acid and genetically produced from rapeseed. Canola oil is obtained by extract sources. Heavy metals are a unique form of toxin because they may exist in the environment for a longer period. Exogenous administration of gibberellic acid has been proven in several studies to increase crop output by affecting an important physiological process. This study of the aimed and objectives included the nickel sulphate stress in quantity 300 µM and gibberellic acid in quantity 0.25 mM in specific quantity and their effects on different plant functions. Super canola under stress condition when 0.25 mM GA3 was applied. Whereas, minimum reduction was observed in cv. Sherilla under stress condition when 0 mM GA3 was applied. Maximum Catalase was observed in cv. Results showed the Peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) are the most important protective enzyme systems in plants, and also the most important reactive oxygen species clearance system. Maximum Total Amino acid was observed in cv. Sherilla under control condition when 0 mM GA3 was applied. However, effect of GA3 application was observed non-significant and same for as varietal difference among two genotypes was observed non-significant. Overall, super canola performed better under stress or non-stress condition. Mustard plants exposed to nickel showed decreased morphological characteristics, which might be related to the degradation of plant architecture, which limits the capacity of water content and minerals absorption by plants, resulting in total development of plant loss.