Abstract: TruScreen is a unique electro-optical technology that provides a direct means of tissue differentiation as a primary screening tool in the general population for cervical cancer and precancerous change (CIN). Objectives of the work are evaluation of sensitivity and specificity of the optoelectronic method in the detection of CIN and cervical cancer. The present study shows correlation between the pNOR number and sensitivity/specificity of the optoelectronic method. The study included 293 patients with abnormal cervical cytology result and the following examinations: examination with the use of the optoelectronic method – Truscreen, colposcopic examination, and histopathologic biopsy. Specificity of the optoelectronic method for LGSIL was estimated at 65.70%, for HGSIL and squamous cell carcinoma of cervix amounted to 90.38%. Specificity of the optoelectronic method used to confirm lack of cervical pathology was estimated at 78.89%. The field under the ROC curve for the optoelectronic method was estimated at 0.88 (95% CI, 0.84–0.92) which shows high diagnostic value of the test in the detection of HGSIL and squamous cell carcinoma. The optoelectronic method is characterized by high usefulness in the detection of CIN, present in the squamous epithelium and squamous cell carcinoma of cervix. In conclusion, we suggest that TruScreen holds the potential to detect lesions that might be missed by cytology alone and clarify unsatisfactory or ASCUS cytology results. With regard to patients, they can benefit from more rapid follow-up and early treatment. We also noticed that the women were happy with the real time results, which are now available using TruScreen. TruScreen technology seems to have a high potential to improve and standardize the screening of the cervical carcinoma.