Sorghum stands as the fifth most valuable global cereal crop, widely grown in semi-arid and arid regions of the world. This present investigation details the variability of polyphenols, scavenging activity in terms of ascorbic acid equivalents of 60 grain sorghum genotypes collected from various countries and were grown at Indian Institute of Millet Research fields which include 38 white, 15 red, and 7 brown pericarp grain sorghum genotypes. Polyphenols range from 575.05 to 3161.87 mg GAE. kg-1, 888.33 to 4230.14 mg GAE.kg-1, 1274.91 to 2885.72 mg GAE. kg-1 in white, red and brown sorghum genotypes respectively. The DPPH radical scavenging activity ranges from 555.74 to 6058.80 mg AAEQ. kg-1, 1190.19 to 6549.59 mg AAEQ.kg-1 and 2174.43 to 5494.40 mg AAEQ. kg-1, in white, red and brown sorghum grain genotypes respectively. Ultra fast liquid chromatography (UFLC) profiling of phenolic acids done in phenolic extracts of red, white and brown sorghum genotypes showed that analytes were derivatives of benzoic acid and cinnamic acid. Total of eleven different phenolic acids were identified, of which ferulic acid is highly expressed in white and red sorghum genotypes only.