Abstract: A novel, sensitive, specific and reliable gradient HPLC (High Performance Liquid Chromatography) method has been developed and validated for the identification and quantification of acids namely, Glyoxalic acid, Glycolic acid, Formic acid, Acetic acid, Diglycolic acid, Chloro acetic acid, Dichloro acetic acid and Trichloro acetic acid. A novel, suitable, rapid and sensitive analytical method was developed using HPLC technique for the above acids quantification and identification. The chromatographic separation of the acids were achieved on Atlantis T3 (250*4.6) mm, 5.0 µ column using 0.15 % TFA (Tri Fluoro Acetic acid) in water (v/v): Solvent mixture (98.5:1.5 v/v) as a mobile phase-A and Acetonitrile: Methanol (90:10%v/v). The solvent mixture was prepared by mixing the acetonitrile and methanol solvents in the ratio of 90:10 volumes. The HPLC method was validated with respect to limit of detection, limit of quantitation, specificity, linearity, accuracy and precision.