Background: Pelvic inflammatory disease (PID) is one of the most serious infections in women. It is an inflammation and infection of the upper genital tract involving the uterus, fallopian tubes, ovaries, and the surrounding structures. Pelvic inflammatory disease (PID) is a global problem, and it is common in both developed and developing countries. It can cause infertility, ectopic pregnancy, and chronic pelvic pain. Risk factors for PID are multiple sexual partners, single status, lower socioeconomic status, young age (<30 years), intrauterine contraceptive device, endometrial biopsy, curettage, hysteroscopy and hysterosalpingography, and Complications of tubal infertility, ectopic pregnancy, chronic pelvic pain. Objective: The aim was to assess the level of nurses’ knowledge and practice regarding pelvic inflammatory disease at Shaheed Ziaur Rahman Medical College Hospital, Bogura. Methodology: This was a descriptive cross-sectional study with a sample size of 115 that was a purposive sampling technique followed by those who met the inclusion criteria to assess the nurse’s knowledge and practice regarding pelvic inflammatory disease. The study was conducted from July 2022 to December 2022. The instruments for data collection were a semi-structured questionnaire composed of three parts: Demographic variables, knowledge, and practice-based information on pelvic inflammatory disease. Results: The findings of the present study in socio-demographics revealed that the highest 64.35% were 31-40 years old, 88.70% were female, 93.91% were married, 93.04% were Muslim, and 61.74% had a Diploma in Nursing and the main findings of average knowledge score 40.00% were the low level of knowledge regarding the pelvic inflammatory disease. It is may not be practical experience in their existing situation. Conclusion: The worldwide increase in the incidence of PID during the last few decades has led to secondary epidemics of tubal factor infertility and ectopic pregnancy. The application of in-service education and training programs will be significant in enhancing the knowledge and self-reported practices among nurses regarding preventive measures of PID.