Tetracera rosiflora Gilg is a medicinal plant used in the Congolese pharmacopeia for the treatment of various diseases. The aim of this work was to achieve the micrographic analysis of the powder of leaves of T. rosiflora from Pumbu City (Kinshasa) and Lukala areas (Kongo Central), to determine their chemical composition and to evaluate the antioxidant activities of their different extracts. Extracts from leaves of this species were characterized for their chemical fingerprint by Thin Layer Chromatography and their in vitro antioxidant activities were evaluated using ABTS, DPPH assays. Microscopically leaves can be characterised by non-glandular trichomes, sinuous anticlinal epidermal cells, and parenchymal cells with cristal of calcium oxalate, polycytic stomata and spiraled vessels. Thin Layer Chromatography showed that leaves of Tetracera rosiflora contain varied phytochemicals such as anthocyanins, flavonoids, coumarins, iridoids, phenolic acid, tanins and the terpenes. All tested extracts (methanolic and dichloromethane) have exhibited high antiradical activities. The obtained results showed that the interesting bioactivities of T. rosiflora correlated with the chemical composition, which depends on several factors including climatic conditions. T. rosiflora is a phytogenetic resource of the Congolese pharmacopoeia with promising therapeutic potentials for the management of various pathologies associated with oxidative stress.