Road Safety Audit (RSA) is a formal procedure for assessing accident potential and safety performance of new and existing roads. RSA is an efficient, cost effective and proactive approach to improve road safety. It is proved that RSA has the potential to save lives. RSA appears to be an ideal tool for improving road safety in India, as basic and accurate data on accidents have yet to be collected. The study aims to evaluate Road Safety Audit of a section of four - lane Madurai - Chennai, National Highway (NH) - 45 and will focus on evaluating the benefits of the proposed actions that have emanated from deficiencies identified through the audit process. After conducting RSA, it is found that trucks are parked on highway which reduces the effective width of carriageway and creating traffic hazards to high speed moving traffics. Unauthorized median openings were found which should be immediately closed. Missing road and median markings to be done and speed signs should match with speed. Access and service lanes are also in deficit which requires immediate improvement. The most Vulnerable Road User (VRU) i.e. pedestrians and cyclists facilities near habitations are lacking and needs to be facilitated on priority.