Cassia angustifolia is a plant best known for its medicinal properties, commonly known as Indian Senna. This is a flowering plant belonging to the Fabaceae family. In this Study, the antibacterial activities of ethanolic extract of Cassia angustifolia leaves were evaluated against MDR Microorganisms. The antimicrobial activity of the extract was assessed against a range of bacterial strains, including Escherichia coli (MDR), Klebsiella sp. (MDR), Pseudomonas sp. (MDR), and Acinetobacter sp. (MDR). The result showed that the leaf extract of Cassia angustifolia exhibit significant antimicrobial activity against all tested MDR Microorganisms. The phytochemical content of the plant includes many bioactive compounds like Tannins, Saponins, Flavonoids, Alkaloids, Steroids and quinones etc which may be responsible for the observed antimicrobial activities. These findings suggest that Indian Senna, which could be used to develop new drugs for the treatment of resistant bacterial infections.