Purpose: The study aimed to identify the Musculoskeletal problem among the third-gender population in Dhaka. Objective: This study’s objective was to find out socio-demographic information, and Musculoskeletal problems among the third-gender population in Dhaka. Methodology: This study was performed in a cross-sectional study design. This study was conducted to determine the Musculoskeletal problem among the third-gender population. This study’s sample was collected through a convenience sampling procedure and a total sample of 62. The data was collected from the different areas across Dhaka. The data collection process was a questionnaire with a face-to-face interview. Data was analyzed with Microsoft Office, and Excel 2019 using the SPSS 25 version software program and test use of study chi- square test. Result: This study’s participant means and standard deviation of participant age where are Mean ±SD= 30.90±10.710; About (45%) third gender age 18-27 years; (32%) age 28-37 years; (12%) age 38-47 years; and 11% more than 47 years. 72.6% were male sex at birth and 27.4% were female sex at birth. Around (14.50%) of them live in rural areas, 4.80%) are semi-urban and (80.60%) are from urban areas. (53.2%) were experiencing pain in the different body parts. 21.2% neck pain, 15.2% shoulder pain, 6.1% elbow pain, 39.4% knee, and 39.4% hip pain. severity level was mild pain felt at 8.10%, moderate pain experienced at 27.40% and 17.70% suffered from severe pain. Conclusion: The database revealed a significant prevalence of musculoskeletal issues (53.2%), primarily affecting the hip and knee. To enhance accuracy, employing a standardized observation tool is recommended. Further research, including larger samples among the third gender in Bangladesh, is essential for robust decision-making and increased awareness.