Background: Male infertility is a significant yet under-reported public health concern. It affects 30% of couples who regularly engage in unprotected sexual activity. In Nigeria, the male partner is sometimes unwilling to have a fertility evaluation, which results in the underreporting of male infertility. The female partner is frequently held responsible for infertility. Hyperprolactinaemia has been identified as a reversible cause of male infertility, which is amenable to treatment, but studies to evaluate the concept are sparse. Aim: To determine if there is an association between high prolactin levels and abnormal seminal fluid analysis parameters. Methods: The study is a cross-sectional analytical study involving male partners of infertile couples. Two hundred and thirty-three (233) patients that met the inclusion criteria and gave informed consent were enrolled on the study at the Human Reproduction and Research Program Unit (HRRP) of the University of Benin Teaching Hospital, Benin-city, Nigeria. A detailed history was taken, and a physical examination was done for all subjects. Seminal fluid analysis was done for all subjects, and blood was also collected for serum prolactin assay. Information was obtained from sociodemographic data and medical history. Data obtained using interviewer-administered questionnaires were analyzed with SPSS Package version 20.0. Results: The study included 233 patients, with a mean age of 40.77.1 and a majority (57.7%) of the subjects with aberrant SFA between the ages of 40 and 49. Participants with normal and abnormal SFA had hyperprolactinemia at 4.3% and 14.2%, respectively. There was a significant negative correlation between prolactin level, motility (r=-0.010, p=0.001), morphology (r=-0.077, p=0.001) and sperm count (r=-0.082, p=0.003). Obesity, alcohol consumption and smoking were significant predictors of abnormal sperm parameters (p=0.011, p=0.001, and p=0.001, respectively). Conclusion: This study indicated a relationship between hyperprolactinaemia, sperm count, motility, and morphology, which suggests that increased prolactin may negatively affect semen quality if left untreated.