Bandar-e-Anzali is located among many active faults in Guilan Province in the north of Iran, and seismologically is one of the active regions of Iran. Numerous severe historical and instrumental earthquakes, including three earthquakes with magnitude more than 7 in this region, can be an evidence of this claim. The objective of this paper is to estimate acceleration coefficient for different levels of seismic activities within the city surrounding area. For this purpose, a list of major faults, as well as the reported historical and instrumental earthquakes occurred within a radius of 200 km around the area, till 2013 A.D. are collected. In this paper, after culling and removing aftershocks and foreshocks, first the Poisson behavior of the remaining earthquakes is studied; and then by calculating the frequency of earthquakes and using universal probabilistic relationships, the seismicity parameters of this region is obtained. Finally, the site-specific response spectra and the peak ground acceleration coefficients for this city and suburbs are obtained using SeisRisk III hazard analysis program. The results of this study show that the active seismicity in the south area of this city is much more than its northern parts.