The purpose of this study is mainly to analyze the influence of sports volunteers’ participation motivation and job satisfaction on re-participation intention. Students who volunteer to participate in the volunteer work of the 2023 National Intercollegiate Athletic Games are the research objects. A questionnaire survey is conducted at each site during the competition. 200 questionnaires are distributed, with 13 invalid questionnaires and 187 valid questionnaires collected. The effective questionnaire recovery rate is 93%. This research is analyzed by statistical methods, such as narrative statistics and multiple regression, and so forth. The study has the following findings: In the volunteer service of the National Intercollegiate Athletic Games, the participation motivation of the university sports volunteers is the highest in “the method and techniques of learning to do things”. In terms of job satisfaction, the highest is “Feel that I am part of the school". Therefore, in the process of volunteering service, sports volunteers feel a high degree of job satisfaction, and their intention to participate in volunteer service in the future is consequently higher. Based on the above results, this study not only provides specific suggestions for planning volunteer services for large-scale sports events in the future, but also proposes directions for future research.