Background: Preterm labor is one of the biggest health issues in the globe and is associated with high perinatal mortality and morbidity. A healthy pregnancy can turn into one that is at high risk for both the mother and the fetus in one of these clinical scenarios. Objective: The aim of the present study is to determine the risk factor and maternal and perinatal outcome of patients presenting with preterm labor and to evaluate the preventable causes of preterm labour. Methods: A Case control study conducted in the Department of Gynaecology and Obstetrics Dhaka Medical College Hospital, during February 2010 to July 2010. Results: A total of 50 cases having preterm labour in between 28 - 36 weeks of gestation and 50 controls having 37-40 weeks pregnancy with lobour pain were screened and enrolled in the study. Several significant risk factors were associated with preterm labour and the following risk factors were identified. Low socio-economic class, rupture of membrane (OR = -2.45), previous history of abortion (OR = 2.25), Preterm delivery (OR -9.33), and Anaemia (OR = 8.31). Conclusions: As preterm labour is grave consequences for both the mother and the newborn of preterm labor should be aim and developing awareness of pregnant mother who are high risk for preterm labor.