Background: The prevalence and impact of nosocomial infections, the importance of infection control measures, and the role of nurses in preventing and managing these infections. It could also discuss previous research on the topic and identify gaps in knowledge or areas for further investigation. Nosocomial infections challenge healthcare and cause prolonged recovery and death if untreated. Bacteria, fungi, and viruses can cause these infections. Objective: The study also aimed to highlight the importance of continuous education for registered nurses to improve their efficiency in preventing nosocomial infections. Material and Method: This descriptive observational study was conducted at Rajshahi Medical College Hospital, Rajshahi, in Bangladesh, from July to November 2019. The relationships between nurse’s knowledge and practice, with a sample size of 200 nurse’s. The age range of the participants was 30-35 years (40%), 36-40 years (20%), 41-45 years (16%), 46-50 years (16%), and 50+ years (8%). Females constituted 89% of the sample, with 76% having a diploma in nursing and midwifery, 12% having a B.Sc. in nursing, and 12% having an M.Sc./MPH. Result: According to a survey of nurse’s, 86% knew about nosocomial infections, with 74% citing improper sterilization as the main cause and 66% aware of risk factors. Most believed they spread in hospitals (64%), and poor health status was a primary risk factor (64%), with 92% received health education, and 98% received help with investigations. Tuberculosis was the most common organism responsible (60%) followed by Candida albicans (26%). Primary risk factors were poor health status (64%) and surgical drains (10%). Disinfection of patient equipment (72%) was the most effective barrier, and urinary tract infections (66%) were the most common complication. Environmental changes (70%) were considered the best prevention method. Conclusion: The study highlights the need for registered nurses to stay up-to-date with infection control and sterile technique principles to prevent nosocomial infections. To effectively care for both the mother and the newborn, it is necessary to determine the best approach to them both for the entirety of the pregnancy.