In Sudan, invasive Cervical cancer is the one of the most common causes of death. The Herpes simplex virus ( HSV-2) is second common causes of the invasive cervical cancer , with fraction progress to cervical cancer disease pointing to possible the role of additional risk factors in development of disease which include: host genetic factor and other infection such as HPV-16, for that women should have regular cervical screening test to detect early cervical cancer. Materials & Methods: This is a retrospective cross sectional study that includes about 75 embedded paraffine wax tissue blocks using immune histochemical stain from patients previously diagnosed as cervical cancer and expression of herpes simplex virus. Details of the patients information taken from medical reports .The analysis done by SPSS. Result: The mean age in this study is 60 with group range between (61-70) years (83.3 %), The Herpes Simplex virus is express only in squamous cell carcinoma. Discussion: The mean age is 60 with age group (61-70) (n= 5: 20%) expression of HSV-2 with (P= 0.136), and the expression of HSV-2 with squamous cell carcinoma of tumor type (n= 6:10.90%) with (P= 0.476), histological grade well differentiated (n=6:11.10%) with (P= 0.477). Conclusion: The expression of HSV-2 was insignificant in age group. The expression of HSV-2 was insignificant in tumor type squamous cell carcinoma. The expression of HSV-2 was insignificant in histological grade.