Background: Hundreds of thousands around the world die from a disease caused by smoking cigarettes. Cigarette smoking (CS) is considered a worldwide major cause of preventable morbidity and mortality. The main clinical consequences of prolonged exposure to CS are chronic respiratory diseases, increased incidence of a variety of cancers, cigarette smoking had a dangerous effect on the essential biochemical mechanisms on the human body. Objective: This study aimed to assess the liver function in Sudanese male cigarettes smoker to identify the influence of cigarettes smoking on the level of their parameters. Material and methods: The study was designed as case control and include 81 samples, which is divided into case (51 samples) and control group (30 samples). The data collected by the use of questionnaire and blood specimens, and the levels of the parameters is measured by A15 automation spectrophotometer. Then the collected data is analyzed by the use of SPSS. Results: The results of the study showed statistically significant increase in total bilirubin and liver enzymes in case group when compared with control group. The mean of plasma total bilirubin, GOT (AST), GPT (ALT), and ALP levels in case group is (0.663, 26.49, 18.82 and 159.27) and in control group is (0.543, 23.00, 14.30 and 146.63) with p. value (0.001, 0.041, 0.004 and 0.000) respectively. And showed statistically insignificant decrease in total protein and albumin in case group when compared with control group, the means of total protein and albumin in case group is (66.47 and 33.73) and in control group is (68.30 and 34.43) with p. value (0.322 and 0.403) respectively. Conclusion: The study concluded that there was significant increase in liver enzymes (GOT, GPT, and ALP) and total bilirubin in cigarette smoking. And significant decrease in total protein and albumin, in cigarette smoking when compared to nonsmoking.