Naked eye single tube red cell osmotic fragility test (NESTROFT) is a simple test to screen for thalassemia has been described for use in developing countries. We studied the articles published before regarding the use of NESTROFT in screening Thalassemia and found out the average sensitivity and negative predictive value was 95.94% and 95.33% respectively which indicates NESTROFT is a good screening tool for the beta thalassemia.