Objective: Metal based nanomaterials has been implicated in the treatment and healing of antibiotic – resistant bacteria infected wounds such as found in patients with tuberculosis, human immunodeficiency virus (HIV) and diabetes. Current research investigates the wound healing potentiation of green synthesized silver nanoparticles in the presence of Gentamicin in Wistar rats. Methods: The reaction of phytochemical extracts of Anonna muricata and aqueous solution of silver nitrate afforded the silver nanoparticles used in this study. The silver nanoparticles produced were subsequently characterized using advanced spectroscopic techniques including; Transmission electron microscopy, Ultra-violet visible spectroscopy and Photon correlation spectroscopy (DLS). Adult Wistar rats were divided into five (5) groups of three rats each. Excision wound (3.0 mm diameter) were made on the dorsal part of each rat under anesthesia. Topical application of various test formulation were administered on the excision wound twice daily and wound healing parameters such as % wound contraction, re-epithelialization time and scare formation were monitored over a period of 12 days . Group 1 received simple ointment base, group 2 received 0.01 % w/w penicillin in ointment base, group 3 received 0.01 %w/w gentamicin in ointment base only, group 4 received 1.0 % w/w silver nanoparticles in ointment base, while group 5 received a combination of 1.0 % w/w silver nanoparticles and 0.01 % w/w gentamicin. Results: The mean particle size of the bio-synthesized silver nanoparticle were found to be 24 ± 1.0 nm. There are observed synergy in wound healing effect amongst the combination of silver nanoparticles and gentamicin after 12 days with respect to the % wound contraction, re-epithelialization time and scare formation when compared to single formulation and controls. Conclusion: Improved wound healing could be achieved using nanotechnology.