Aim: To evaluate the effect of two different activation protocols of periodontal distractor on periodontal health after rabid canine retraction. Objectives: 1) to evaluate the periodontal health of two activations/day of periodontal distractor. 2) To evaluate the periodontal health of four activations/day of periodontal distractor. 3) To compare between two different activation protocols of periodontal distractor on periodontal health. Methods: The study was carried out on 32 canine of 16 female patients (16-22 years of age) requiring extraction of bilateral maxillary first premolars and maximum anchorage. They were divided into two groups. (Group I): Canine retraction was done by periodontal distractor with twice activations per day. (Group II): Canine retraction was done by periodontal distractor with four activations per day. Periodontal distractor was cemented after bilateral maxillary premolar extraction and inter septal bone corticotomy was done. The periodontal health of maxillary canines was evaluated using these parameters before and after retraction: (gingival index, periodontal index and probing depth). All data were explored for normality by using Shapiro Wilk and Kolmogorov Normality test which revealed that all data originated from non-parametric data. Results: for periodontal index and probing depth there was insignificant difference between them in before and after in both groups. For gingival index there was insignificant difference between them in before, while in after group II was significantly higher than group I. Conclusion: In this study, there was increase in all periodontal health parameters including (gingival index, periodontal index and probing depth) after using of periodontal distractor with both protocols of activations denoting gingivitis. The 2 activations/day was better than 4 activations/day for periodontal health during canine retraction by periodontal distractor.