Etiological agents causing infection in the nails vary with gender, age, occupation, A total number of 51 patients having clinically diagnosed Onychomycosis were selected, Direct microscopy of nail specimen in 40% KOH, culture on SDA, DTM was done. out of 51 patients, 17 were between 20-30 years of age (33.33%),out of 51 patients 33 were males and 18 were females, 27 patients (52.94%) had DLSO pattern, Of the 32 positive cultures, Dermatophytes were isolated in 10 (23.80%) and Non-Dermatophytes in 15 (29.41%). Non-Dermatophyte fungi are being isolated more frequently from nail infections.