Background: Helicobacter Pylori (H. pylori) infection is a major gastric infection worldwide and has been associated with many hematologic disorders. Objective: The purpose of this study was to determine the effect of H. pylori infection on Sudanese people. Method: Blood samples are collected from 60 persons; 30 patient (as sample) and 30 patients (as control sample). Vitamin B12 and complete blood count (CBC) tests are performed. Results: The results revealed that Vitamin B 12 level mean was significantly lower among H. pylori patients than the controls (p-value 0.013). The results also revealed that there are no significant differences between gander in vitamin B12 level among patients. The correlation between vitamin B12 and age was found to be negative (R=0.444 & P=0.014). Conclusion & Recommendations: Vitamin B12 level, HB and RBC are lower in H. pylori infected Sudanese individuals compared to the normal people. Thus, we highly recommend for further research and case-control studies in discovering the causal factors related to reduction of vitamin B12 among H. pylori infected Sudanese individuals.