Introduction: Urinary tract infection (UTI) is the third most common infection during childhood and considered as an important risk factor for the development long-term complications. It was aimed to look into the demographic characteristics, clinical presentations, and antimicrobial resistance among children hospitalized for UTI. Methods: This cross-sectional observational study was conducted at the Inpatients Department of Uttara Adhunik Medical College and Hospital, Dhaka, Bangladesh. The study duration was 2 years, from July 2017 to June 2019, during which period, a total of 90 children with urinary tract infection visiting the study place were included in the study, following the inclusion and exclusion criteria. Result: The age of the participants ranged from over 1 months to 5 years., with majority (55.56%) being between the age of 2-5 years. The male to female ratio was 1:1.73. Fever was the commonest presenting symptom in participants, with 87.78% prevalence, followed by vomiting in 80% of the participants. The commonest organism cultured in this study was E. coli (80%), followed by Klebsiella spp. (20%). Isolated pathogens were highly sensitive to Amikacin (100.0%), Meropenem (100.0%), Imipenem (97.0%) and Nitrofurantoin (90.9% - 100.0%), moderately sensitivity to third generation Cephalosporine and highly resistant to Ampicillin (75.0 - 78.8%), Amoxiclav (72.7% - 87.5%) and Cephalexin (81.1% - 87.5%). Conclusion: The proportion of urinary tract infection in females was higher than males. Most patients presented with more than one symptom but most commonly presented with fever. E. coli was the most common isolated bacteria in hospitalized children with a principal diagnosis of UTI. Most of the isolated pathogens were highly resistant to ampicillin, cotrimoxazole, and highly sensitivity to amikacin, nitrofurantoin and meropenem.