Background: Gallstones are the major culprits for gallbladder pathologies. More than 95% of biliary tract disease is attributable to cholelithiasis (gallstones). Most patients are unaware of the disease and remain asymptomatic for life. Aim: To determine morphological characterization of gallstones in cholecystectomy specimens to understand its association with etiology. Material and Methods: All cholecystectomy specimens having gallstones received at the Department of Pathology over a period of four years were studied for morphological characterization. Results: Out of 178 gallbladders showing calculi, patients of 5thand 7th decade (24.15% and 21.92%) were commonly affected and majority were females (69%) when compared to males (31%). Most common calculi were mixed type of stones (64.04%). 48.88% gallbladders were having round to oval shaped calculi, 24.15% were polyhedral, multifaceted and 26.97% were irregular in shape. Conclusion: The population of the present study showed mixed type of calculi as common observation which depicts its etiology of change in lifestyle with following western diets and concurrent infectious cause.