Candida continues to be leading cause of morbidity and mortality in large population of immunocompromised and hospitalized patients. Invasive Candidiasis due to non-albicans candida has been on the rise in last few years. Incidence rates vary geographically, often because of different patient populations studied. The present study was conducted to find out the species distribution and antifungal susceptibility of Candida species from different sources and patient population of our tertiary care hospital. A total of 103 Candida species were isolated from the different clinical specimens of suspected candida infection cases, In this study, it was observed that candidiasis can occur at all ages and in both sexes. 82 (79.6%) isolates were obtained from cases admitted in different inpatient departments of which 51 (49.5%) accounted for isolates from various ICUs mainly NICU. Most of the isolates obtained were from urine samples (44.6%) followed by blood (34.9%). Non albicans Candida were isolated at a higher rate (52.8%) than Candida albicans (47.5%). Among all species of Candida commonest isolate was C. albicans (47.5%) followed by C. tropicalis (26.21%). Overall high suceptibility to voriconazole and Amphotericin B (99.03%). This study emphasizes the need for monitoring local epidemiologic data and antifungal susceptibility pattern of candida isolates for proper treatment.