Introduction: Pemphigus vulgaris is an rare Autoimmune blistering disease. The common cause of death in Pemphigus is septicemia which is secondary to cutaneous bacterial infection. Aim: The aim of this study is to find out bacteriological profile and its antimicrobial susceptibility pattern in patients with Pemphigus vulgaris attending tertiary care hospital, Jamnagar. Materials and Methods: The present study was conducted using the 198 skin swab culture and sensitivity reports collected retrospectively in department of Microbiology Shri M.P.Shah Medical College, Jamnagar from July - 2018 to July – 2019. Results: During the study 198 culture Reports were analysed. Out of this 91(45%) were culture Positive. Staphylococcus aureus (65.93%) was the most common organism isolated Followed by the Pseudomonas aeruginosa (17.58%), Klebsiella (8.79%). Other isolates include species of CONS, Escherichia coli, Enterobacter and Acinetobacter (7.69%). Out of this High sensitivity rate were observed against linezolid in gram positive cocci and against Imipenem and Piperacilin/ tazobactum in gram Negative bacilli. Conclusion: Secondary Cuteneous bacterial infection in pemphigus vulgaris is fatal. The Most common Organism is Staphylococcus aureus followed by Pseudomonas aeruginosa, Klebsiella and species of E.coli, acenatobacter and enterobacter showing high resistant to routine antibiotics.