The co-infection with Hepatitis B&C is a common problem in HIV infected patients as immune deficiency accelerates disease progression and may complicate the management of patients. The Co-infection is also associated with the reduced survival and an increases risk of progression to severe liver diseases with higher susceptibility towards hepatotoxicity due to antiretroviral therapy. The co-existence is quite permissive due to their shared modes of transmissions which are mostly through sexual contact with infected individuals, infected blood and blood products and vertical transmission. Materials and Methods: Serum samples were collected from HIV positive patients for detection HBsAg and anti-HCV antibodies by ELISA. Results: Out of 1000 HIV Seropositive patients 45 (4.5%) were positive for Hepatitis B, and 8(0.8%) were positive for HBV and HCV co-infection. Conclusion: Hepatitis B&C co-infection was found to be significantly higher in HIV positive individuals in comparison to normal population. Hepatitis virus infection leads to rapid progression of liver cirrhosis in HIV infected patients. Hence all HIV patients need to be routinely tested for markers of Hepatitis B&C infection may be required to monitor the clinical outcome.