Background: Body fluid samples are routinely received for cytological examination to diagnose inflammatory, benign or malignancy. Diagnostic efficiency depending on the type of fixatives used. Therefore these fluid samples are processed with six different fixatives to study thecytomorphological changes. Aim and objectives: 1.The aim of the study was to study as closely as possible the cytomorphological characteristics of body fluids by different fixatives. 2. To compare and analyse the most effective fixative. Materials and method: Inpresent study, the body fluid samples were received from various out-patient and inpatient departments of saveetha medical college and hospital (from Jan 2019 to March 2019). About 50 body fluid samples of various patients is collected for morphological examination .The moderate amount of fluid (10ml to 15ml) were processed by centrifugation, then smeared and stained. Results: Schaudins and carnoys fixatives are the best among the other six different fixatives which had an excellent nuclear and cytoplasmic features and clear background. Conclusion: Isopropyl alcohol using as an ideal fixative in ctytology laboratory. But Schaudins and carnoys are also as best fixative as that of isopropyl alcohol in body fluid cytology