Objectives: The aim of this study is to compare two different methods in identifying significant bacteriuria. Materials and Methods: A cross-sectional study was conducted for a period of three months from January to March 2019. Urine samples received to Clinical Microbiology Laboratory were subjected to Standard loop technique and Pour plate technique.104 CFU/ml was considered as significant bacteriuria. Statistical analysis was made by chi-square test. Results: Out of 300 urine samples, 87 samples were shown to have Significant Bacteriuriaby Standard loop technique, 91 samples were shown to have Significant Bacteriuria by Pour plate technique. The Escherichia coli accounts for (30%) of isolates causing Significant Bacteriuria followed by Proteus species (17%), Enterococcus species (16%), and Pseudomonas species (14%). Male preponderance was observed over females. The Pour plate technique was found to be most sensitive method in identifying significant Bacteriuria. Conclusion: The Pour plate technique was observed to be the sensitive method in identifying significant bacteriuria compared to standard loop technique. Escherichia coli showed the highest rate of isolation [1]. Multi-drug resistance is seen in Klebsiella species.