Urinary tract infections are defined as the spectrum of diseases of the genito urinary tract by micro organisms. The risk of urinary tract infection (UTI) is higher in diabetics and the antibiotic resistance of uropathogens have been changing over the past few years. Hence, the study was undertaken to determine the prevalence of various uropathogens and their antibiotic sensitivity pattern in diabetic patients with UTI. Methods: Clean voided midstream urine was collected, & inoculated on Blood Agar (BA) and McConckey Agar (MA), and incubated at 37OC overnight for visible growth. Results: Out of 120 diabetic patients, 75 patients (30 males and 45 females) were found to have culture positive UTI. The rate of isolation of Klebsiella species (50%) was highest, followed by Escherichia coli (16.6%), Staphylococcus aureus (16.6%), Coagulase negative Staphylococcus (12.5%) and Citrobacter species (4%). All the isolated organisms showed maximum sensitivity to Carbapenems and least susceptibility to Norfloxacin and Nitrofurantoin. Conclusion: High blood glucose is the main cause of UTI among diabetics. Therefore, isolation of pathogens in diabetic patients with UTI is important to initiate appropriate antibiotic therapy and to prevent renal complications